ADK antibody (AA 165-351)

Catalog No. ABIN7600222

The Mouse Monoclonal anti-ADK antibody has been validated for WB, IHC and FACS. It is suitable to detect ADK in samples from Human.

Quick Overview for ADK antibody (AA 165-351) (ABIN7600222)

Target: ADK (Adenosine Kinase (ADK))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ADK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 7F4

Product Details anti-ADK Antibody

Binding Specificity: AA 165-351

Purpose: Anti-ADK Antibody Picoband® (monoclonal, 7F4)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ADK Antibody Picoband® (monoclonal, 7F4) (ABIN7600222). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human ADK recombinant protein (Position: K165-T351). Human ADK shares 88.8% and 88.2% amino acid (aa) sequence identity with mouse and rat ADK, respectively.

Isotype: IgG1

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
1. Boison D, Scheurer L, Zumsteg V, Rulicke T, Litynski P, Fowler B, Brandner S, Mohler H: Neonatal hepatic steatosis by disruption of the adenosine kinase gene. Proc Natl Acad Sci U S A 2002, 99: 6985-6990. 2. Fox IH, Kelley WN: The role of adenosine and 2'-deoxyadenosine in mammalian cells. Annu Rev Biochem 1978, 47: 655-686.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ADK

Target: ADK (Adenosine Kinase (ADK))

Alternative Name: ADK

Background:

Synonyms: Adenosine kinase, AK, Adenosine 5'-phosphotransferase, ADK

Tissue Specificity: Widely expressed. Highest level in placenta, liver, muscle and kidney.

Background: This gene is an enzyme which catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 45 kDa

Gene ID: 132

UniProt: P55263

Pathways: Ribonucleoside Biosynthetic Process