RANGAP1 antibody (AA 165-587)

Catalog No. ABIN7600225

The Rabbit Polyclonal anti-RANGAP1 antibody has been validated for WB, IHC, IF, ELISA, FACS and ICC. It is suitable to detect RANGAP1 in samples from Human, Mouse and Rat.

Quick Overview for RANGAP1 antibody (AA 165-587) (ABIN7600225)

Target: RANGAP1 (Ran GTPase Activating Protein 1 (RANGAP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RANGAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-RANGAP1 Antibody

Binding Specificity: AA 165-587

Purpose: Anti-RANGAP1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RANGAP1 Antibody Picoband® (ABIN7600225). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RANGAP1 recombinant protein (Position: A165-V587).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bernier-Villamor, V., Sampson, D. A., Matunis, M. J., Lima, C. D. Structural basis for E2-mediated SUMO conjugation revealed by a complex between ubiquitin-conjugating enzyme Ubc9 and RanGAP1. Cell 108: 345-356, 2002. 2. Bischoff, F. R., Klebe, C., Kretschmer, J., Wittinghofer, A., Ponstingl, H. RanGAP1 induces GTPase activity of nuclear Ras-related Ran. Proc. Nat. Acad. Sci. 91: 2587-2591, 1994. 3. Bischoff, F. R., Krebber, H., Kempf, T., Hermes, I., Ponstingl, H. Human RanGTPase-activating protein RanGAP1 is a homologue of yeast Rna1p involved in mRNA processing and transport. Proc. Nat. Acad. Sci. 92: 1749-1753, 1995.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RANGAP1

Target: RANGAP1 (Ran GTPase Activating Protein 1 (RANGAP1))

Alternative Name: RANGAP1

Background:

Synonyms: CD3 antigen, epsilon polypeptide, CD3e molecule, Cd3e, Cd3e_predicted, rCG_58471

Background: Ran GTPase-activating protein 1 is an enzyme that in humans is encoded by the RANGAP1 gene. This gene encodes a protein that associates with the nuclear pore complex and participates in the regulation of nuclear transport. The encoded protein interacts with Ras-related nuclear protein 1 (RAN) and regulates guanosine triphosphate (GTP)-binding and exchange. Alternative splicing results in multiple transcript variants.

Molecular Weight: 70 kDa

Gene ID: 5905

UniProt: P46060

Pathways: M Phase, Protein targeting to Nucleus