Calmegin antibody (AA 18-611)

Catalog No. ABIN7600354

The Rabbit Polyclonal anti-Calmegin antibody has been validated for WB and ELISA. It is suitable to detect Calmegin in samples from Mouse and Rat.

Quick Overview for Calmegin antibody (AA 18-611) (ABIN7600354)

Target: Calmegin (CLGN)

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calmegin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Calmegin Antibody

Binding Specificity: AA 18-611

Purpose: Anti-Calmegin/Clgn Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Calmegin/Clgn Antibody Picoband® (ABIN7600354). Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Calmegin/Clgn recombinant protein (Position: N18-D611).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Ikawa, M., Nakanishi, T., Yamada, S., Wada, I., Kominami, K., Tanaka, H., Nozaki, M., Nishimune, Y., Okabe, M. Calmegin is required for fertilin alpha/beta heterodimerization and sperm fertility. Dev. Biol. 240: 254-261, 2001. 2. Ikawa, M., Wada, I., Kominami, K., Watanabe, D., Toshimori, K., Nishimune, Y., Okabe, M. The putative chaperone calmegin is required for sperm fertility. Nature 387: 607-611, 1997. 3. Tanaka, H., Ikawa, M., Tsuchida, J., Nozaki, M., Suzuki, M., Fujiwara, T., Okabe, M., Nishimune, Y. Cloning and characterization of the human calmegin gene encoding putative testis-specific chaperone. Gene 204: 159-163, 1997.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Calmegin

Target: Calmegin (CLGN)

Alternative Name: Clgn

Background:

Synonyms: Transcription factor MafA, Pancreatic beta-cell-specific transcriptional activator, RIPE3b1 factor, V-maf musculoaponeurotic fibrosarcoma oncogene homolog A, MAFA

Tissue Specificity: Preferentially expressed in regulatory T-cells (Tregs).

Background: Calmegin, also known as CLGN, is a protein which in humans is encoded by the CLGN gene. This gene belongs to the calreticulin family, which includes calreticulin, calnexin, and calmegin, and encodes a calcium-binding molecular chaperone specifically expressed in pachytene stage male germ cells. It is required for the proper folding of newly synthesized membrane proteins in the endoplasmic reticulum including those critical for sperm migration from the uterus into the oviduct and sperm adhesion to and penetration of the zona pellucida. This gene plays a key role in spermatogenesis and male infertility. Alternative splice variants exist for this gene.

Molecular Weight: 95 kDa

Gene ID: 12745

UniProt: P52194