RRN3 antibody (AA 184-651)

Catalog No. ABIN7600380

This Rabbit Polyclonal antibody specifically detects RRN3 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for RRN3 antibody (AA 184-651) (ABIN7600380)

Target: RRN3 (RRN3 RNA Polymerase I Transcription Factor (RRN3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RRN3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-RRN3 Antibody

Binding Specificity: AA 184-651

Purpose: Anti-RRN3 Antibody Picoband®

Characteristics: Anti-RRN3 Antibody Picoband® (ABIN7600380). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RRN3 recombinant protein (Position: D184-L651). Human RRN3 shares 87.4% amino acid (aa) sequence identity with mouse RRN3.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Mayer, C., Bierhoff, H., Grummt, I. The nucleolus as a stress sensor: JNK2 inactivates the transcription factor TIF-IA and down-regulates rRNA synthesis. Genes Dev. 19: 933-941, 2005. 2. Moorefield, B., Greene, E. A., Reeder, R. H. RNA polymerase I transcription factor Rrn3 is functionally conserved between yeast and human. Proc. Nat. Acad. Sci. 97: 4724-4729, 2000. 3. Yuan, X., Zhou, Y., Casanova, E., Chai, M., Kiss, E., Grone, H.-J., Schutz, G., Grummt, I. Genetic inactivation of the transcription factor TIF-IA leads to nucleolar disruption, cell cycle arrest, and p53-mediated apoptosis. Molec. Cell 19: 77-87, 2005.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RRN3

Target: RRN3 (RRN3 RNA Polymerase I Transcription Factor (RRN3))

Alternative Name: RRN3

Background: RNA polymerase I-specific transcription initiation factor RRN3 is an enzyme that in humans is encoded by the RRN3 gene. This gene encodes a member of the cytochrome P450 superfamily of enzymes, which participate in drug metabolism and the synthesis of cholesterol, steroids and other lipids. This enzyme hydroxylates testosterone and dehydroepiandrosterone 3-sulphate, which is involved in the formation of estriol during pregnancy. This gene is part of a cluster of related genes on chromosome 7q21.1.

Molecular Weight: 74 kDa

Gene ID: 54700

Pathways: Negative Regulation of intrinsic apoptotic Signaling