STAMBPL1 antibody (AA 187-436)

Catalog No. ABIN7600389

This Rabbit Polyclonal antibody specifically detects STAMBPL1 in WB, FACS and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for STAMBPL1 antibody (AA 187-436) (ABIN7600389)

Target: STAMBPL1 (STAM Binding Protein-Like 1 (STAMBPL1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STAMBPL1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Product Details anti-STAMBPL1 Antibody

Binding Specificity: AA 187-436

Purpose: Anti-AMSH-LP/STAMBPL1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-AMSH-LP/STAMBPL1 Antibody Picoband® (ABIN7600389). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human AMSH-LP/STAMBPL1 recombinant protein (Position: Q187-R436).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Kikuchi, K., Ishii, N., Asao, H., Sugamura, K. Identification of AMSH-LP containing a Jab1/MPN domain metalloenzyme motif. Biochem. Biophys. Res. Commun. 306: 637-643, 2003. 2. Kitajima, K., Matsumoto, K., Tahara, M., Takahashi, H., Nakamura, T., Nakamura, T. A newly identified AMSH-family protein is specifically expressed in haploid stages of testicular germ cells. Biochem. Biophys. Res. Commun. 309: 135-142, 2003. 3. Nagase, T., Kikuno, R., Ishikawa, K., Hirosawa, M., Ohara, O. Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 7: 65-73, 2000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for STAMBPL1

Target: STAMBPL1 (STAM Binding Protein-Like 1 (STAMBPL1))

Alternative Name: STAMBPL1

Background:

Synonyms: Interleukin-36 alpha, FIL1 epsilon, Interleukin-1 epsilon, IL-1 epsilon, Interleukin-1 family member 6, IL-1F6, IL36A, FIL1E, IL1E, IL1F6

Tissue Specificity: Expressed in immune system and fetal brain, but not in other tissues tested or in multiple hematopoietic cell lines. Predominantly expressed in skin keratinocytes but not in fibroblasts, endothelial cells or melanocytes. Increased in lesional psoriasis skin.

Background: STAMBPL1 (STAM-binding protein-like 1), also known as AMSH-FP, AMSH-LP (associated molecule with the SH3 domain of STAM like protein) or ALMa, is a 436 amino acid protein that belongs to the peptidase M67C family, contains one MPN domain and a JAMM motif that is essential for protease activity. Existing as two alternatively spliced isoforms, STAMBPL1 is a ubiquitously expressed protein that binds two zinc ions per subunit and acts as a zinc metalloprotease that specifically cleaves Lys-63-linked polyubiquitin chains. The gene that encodes STAMBPL1 maps to human chromosome 10q23.31. Chromosome 10 contains over 800 genes and 135 million nucleotides, making up nearly 4.5 % of the human genome. PTEN is an important tumor suppressor gene located on chromosome 10 and, when defective, causes a genetic predisposition to cancer development known as Cowden syndrome. The chromosome 10 encoded gene ERCC6 is important for DNA repair and is linked to Cockayne syndrome which is characterized by extreme photosensitivity and premature aging. Tetrahydrobiopterin deficiency and a number of syndromes involving defective skull and facial bone fusion are also linked to chromosome 10.

Molecular Weight: 50 kDa

Gene ID: 57559