SMCR7L antibody (AA 189-463)

Catalog No. ABIN7600396

The Rabbit Polyclonal anti-SMCR7L antibody has been validated for WB, ELISA, IF, IHC, ICC and FACS. It is suitable to detect SMCR7L in samples from Human, Mouse and Rat.

Quick Overview for SMCR7L antibody (AA 189-463) (ABIN7600396)

Target: SMCR7L (Smith-Magenis Syndrome Chromosome Region, Candidate 7-Like (SMCR7L))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMCR7L antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-SMCR7L Antibody

Binding Specificity: AA 189-463

Purpose: Anti-MIEF1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MIEF1 Antibody Picoband® (ABIN7600396). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MIEF1 recombinant protein (Position: S189-T463).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hartz, P. A. Personal Communication. Baltimore, Md. 10/25/2013. 2. Palmer, C. S., Elgass, K. D., Parton, R. G., Osellame, L. D., Stojanovski, D., Ryan, M. T. Adaptor proteins MiD49 and MiD51 can act independently of Mff and Fis1 in Drp1 recruitment and are specific for mitochondrial fission. J. Biol. Chem. 288: 27584-27593, 2013. 3. Palmer, C. S., Osellame, L. D., Laine, D., Koutsopoulos, O. S., Frazier, A. E., Ryan, M. T. MiD49 and MiD51, new components of the mitochondrial fission machinery. EMBO Rep. 12: 565-573, 2011.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SMCR7L

Target: SMCR7L (Smith-Magenis Syndrome Chromosome Region, Candidate 7-Like (SMCR7L))

Alternative Name: MIEF1

Background:

Synonyms: Disintegrin and metalloproteinase domain-containing protein 28,ADAM 28,3.4.24.-,Epididymal metalloproteinase-like, disintegrin-like, and cysteine-rich protein II,eMDC II,Metalloproteinase-like, disintegrin-like, and cysteine-rich protein L,MDC-L,ADAM28,ADAM23, MDCL,

Tissue Specificity: Expressed predominantly in secondary lymphoid tissues, such as lymph node, spleen, small intestine, stomach, colon, appendix and trachea. The lymphocyte population is responsible for expression of this protein in these tissues. Isoform 2 is expressed preferentially in spleen.

Background: Mitochondrial dynamic protein MID51 (MID51) also known as mitochondrial elongation factor 1 (MIEF1) or Smith-Magenis syndrome chromosome region candidate gene 7 protein-like (SMCR7L) is a protein that in humans is encoded by the SMCR7L gene. The SMCR7L gene codes for a protein that has been called MiD51/MIEF1 and shown to regulate mitochondrial fission by interacting with the proteins Drp1 and FIS1.

Molecular Weight: 51 kDa

Gene ID: 54471