GCHFR antibody (AA 2-84)

Catalog No. ABIN7600511

This anti-GCHFR antibody is a Rabbit Polyclonal antibody detecting GCHFR in ELISA, IHC, WB, IF, ICC and FACS. Suitable for Human, Rat and Mouse.

Quick Overview for GCHFR antibody (AA 2-84) (ABIN7600511)

Target: GCHFR (GTP Cyclohydrolase I Feedback Regulator (GCHFR))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCHFR antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-GCHFR Antibody

Binding Specificity: AA 2-84

Purpose: Anti-GCHFR Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-GCHFR Antibody Picoband® (ABIN7600511). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GCHFR recombinant protein (Position: P2-E84).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Milstien, S., Jaffe, H., Kowlessur, D., Bonner, T. I. Purification and cloning of the GTP cyclohydrolase I feedback regulatory protein, GFRP. J. Biol. Chem. 271: 19743-19751, 1996

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.005 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for GCHFR

Target: GCHFR (GTP Cyclohydrolase I Feedback Regulator (GCHFR))

Alternative Name: GCHFR

Background:

Synonyms: Zinc finger protein 42 homolog, Zfp-42, Reduced expression protein 1, REX-1, hREX-1, Zinc finger protein 754, ZFP42, REX1, ZNF754

Tissue Specificity: Expressed in kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells (at protein level). Expressed in malignant kidney and several carcinoma cell lines (at protein level). Expressed in embryonic stem cells, kidney, epidermal keratinocytes, prostate epithelial cells, bronchial and small airway lung epithelial cells. Expressed in embryonal carcinomas, seminomas, malignant kidney and several carcinoma cell lines.

Background: GTP cyclohydrolase 1 feedback regulatory protein is an enzyme that in humans is encoded by the GCHFR gene. GTP cyclohydrolase I feedback regulatory protein binds to and mediates tetrahydrobiopterin inhibition of GTP cyclohydrolase I. The regulatory protein, GCHFR, consists of a homodimer. It is postulated that GCHFR may play a role in regulating phenylalanine metabolism in the liver and in the production of biogenic amine neurotransmitters and nitric oxide.

Molecular Weight: 12 kDa

Gene ID: 2644

UniProt: P30047