PKN3 antibody (AA 2-889)

Catalog No. ABIN7600512

This Rabbit Polyclonal antibody specifically detects PKN3 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for PKN3 antibody (AA 2-889) (ABIN7600512)

Target: PKN3 (Protein Kinase N3 (PKN3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKN3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-PKN3 Antibody

Binding Specificity: AA 2-889

Purpose: Anti-PKN3 Antibody Picoband®

Characteristics: Anti-PKN3 Antibody Picoband® (ABIN7600512). Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PKN3 recombinant protein (Position: E2-P889). Human PKN3 shares 83.7% amino acid (aa) sequence identity with mouse PKN3.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hartz, P. A. Personal Communication. Baltimore, Md. 1/23/2007. 2. Leenders, F., Mopert, K., Schmiedeknecht, A., Santel, A., Czauderna, F., Aleku, M., Penschuck, S., Dames, S., Sternberger, M., Rohl, T., Wellmann, A., Arnold, W., Giese, K., Kaufmann, J., Klippel, A. PKN3 is required for malignant prostate cell growth downstream of activated PI 3-kinase. EMBO J. 23: 3303-3313, 2004. 3. Oishi, K., Mukai, H., Shibata, H., Takahashi, M., Ona, Y. Identification and characterization of PKN-beta, a novel isoform of protein kinase PKN: expression and arachidonic acid dependency are different from those of PKN-alpha. Biochem. Biophys. Res. Commun. 261: 808-814, 1999.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PKN3

Target: PKN3 (Protein Kinase N3 (PKN3))

Alternative Name: PKN3

Background: Protein kinase N3 is a serine/threonine protein kinase and one of three PKN isoforms (PKN1, PKN2, PKN3) encoded by independent genes. PKN3 has been identified as an effector that mediates malignant prostate cell growth and is activated by PI3-kinase.

Molecular Weight: 99 kDa

Gene ID: 29941

UniProt: Q6P5Z2