MAD2L1BP antibody (AA 20-274)

Catalog No. ABIN7600535

This anti-MAD2L1BP antibody is a Rabbit Polyclonal antibody detecting MAD2L1BP in WB, ELISA and FACS. Suitable for Human.

Quick Overview for MAD2L1BP antibody (AA 20-274) (ABIN7600535)

Target: MAD2L1BP (MAD2L1 Binding Protein (MAD2L1BP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAD2L1BP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-MAD2L1BP Antibody

Binding Specificity: AA 20-274

Purpose: Anti-MAD2L1BP Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MAD2L1BP Antibody Picoband® (ABIN7600535). Tested in ELISA, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MAD2L1BP recombinant protein (Position: Y20-E274).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Alfieri, C., Chang, L., Barford, D. Mechanism for remodelling of the cell cycle checkpoint protein MAD2 by the ATPase TRIP13. Nature 559: 274-278, 2018. 2. Date, D. A., Burrows, A. C., Venere, M., Jackson, M. W., Summers, M. K. Coordinated regulation of p31(Comet) and Mad2 expression is required for cellular proliferation. Cell Cycle 12: 3824-3832, 2013. 3. Eytan, E., Wang, K., Miniowitz-Shemtov, S., Sitry-Shevah, D., Kaisari, S., Yen, T. J., Liu, S.-T., Hershko, A. Disassembly of mitotic checkpoint complexes by the joint action of the AAA-ATPase TRIP13 and p13(comet). Proc. Nat. Acad. Sci. 111: 12019-12024, 2014.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for MAD2L1BP

Target: MAD2L1BP (MAD2L1 Binding Protein (MAD2L1BP))

Alternative Name: MAD2L1BP

Background:

Synonyms: Intraflagellar transport protein 88 homolog, Recessive polycystic kidney disease protein Tg737 homolog, Tetratricopeptide repeat protein 10, TPR repeat protein 10, IFT88, TG737, TTC10

Tissue Specificity: Expressed in the heart, brain, liver, lung, kidney, skeletal muscle and pancreas.

Background: MAD2L1-binding protein is a protein that in humans is encoded by the MAD2L1BP gene. The protein encoded by this gene was identified as a binding protein of the MAD2 mitotic arrest deficient-like 1 (MAD2/MAD2L1). MAD2 is a key component of the spindle checkpoint that delays the onset of anaphase until all the kinetochores are attached to the spindle. This protein may interact with the spindle checkpoint and coordinate cell cycle events in late mitosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Molecular Weight: 37 kDa

Gene ID: 9587

UniProt: Q15013