METT5D1 antibody (AA 200-407)

Catalog No. ABIN7600565

Product Details anti-METT5D1 Antibody

Target: METT5D1 (Methyltransferase 5 Domain Containing 1 (METT5D1))

Binding Specificity: AA 200-407

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This METT5D1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Anti-METT5D1/METTL15 Antibody Picoband®

Characteristics: Anti-METT5D1/METTL15 Antibody Picoband® (ABIN7600565). Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human METT5D1/METTL15 recombinant protein (Position: R200-L407). Human METTL15 shares 87% amino acid (aa) sequence identity with mouse METTL15.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Chen, H., Shi, Z., Guo, J., Chang, K. J., Chen, Q., Yao, C. H., Haigis, M. C., Shi, Y. The human mitochondrial 12S rRNA m4C methyltransferase METTL15 is required for mitochondrial function. J. Biol. Chem. 295: 8505-8513, 2020. 2. Gross, M. B. Personal Communication. Baltimore, Md. 12/19/2019. 3. Itoh, Y., Khawaja, A., Laptev, I., Cipullo, M., Atanassov, I., Sergiev, P., Rorbach, J., Amunts, A. Mechanism of mitoribosomal small subunit biogenesis and preinitiation. Nature 606: 603-608, 2022.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for METT5D1

Target: METT5D1 (Methyltransferase 5 Domain Containing 1 (METT5D1))

Alternative Name: METTL15 (METT5D1 Products)

Background: Predicted to enable rRNA (cytosine-N4-)-methyltransferase activity. Predicted to be involved in rRNA base methylation. Predicted to be located in mitochondrial matrix. Predicted to be integral component of membrane.

Molecular Weight: 46 kDa

Gene ID: 196074

UniProt: P0C7V9