Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC)
Purpose
Anti-PTCD1 Antibody Picoband®
Characteristics
Anti-PTCD1 Antibody Picoband® (ABIN7600570). Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Purification
Immunogen affinity purified.
Immunogen
E.coli-derived human PTCD1 recombinant protein (Position: D203-L601). Human PTCD1 shares 76.2% and 77.8% amino acid (aa) sequence identity with mouse and rat PTCD1, respectively.
PTCD1
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry, 2-5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/mL, - 1. Hartz, P. A. Personal Communication. Baltimore, Md. 8/17/2012. 2. Ishikawa, K., Nagase, T., Suyama, M., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res. 5: 169-176, 1998. 3. Szklarczyk, R., Wanschers, B. F. J., Cuypers, T. D., Esseling, J. J., Riemersma, M., van den Brand, M. A. M., Gloerich, J., Lasonder, E., van den Heuvel, L. P., Nijtmans, L. G., Huynen, M. A. Iterative orthology prediction uncovers new mitochondrial proteins and identifies C12orf62 as the human ortholog of COX14, a protein involved in the assembly of cytochrome c oxidase. Genome Biol. 13: R12, 2012. Note: Electronic Article.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
This gene encodes a mitochondrial protein that binds leucine tRNAs and other mitochondrial RNAs and plays a role in the regulation of translation. Increased expression of this gene results in decreased mitochondrial leucine tRNA levels. Naturally occurring read-through transcription exists between upstream ATP5J2 (ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F2) and this gene.