SMPD1 antibody (AA 204-558)

Catalog No. ABIN7600575

This Rabbit Polyclonal antibody specifically detects SMPD1 in WB and ELISA. It exhibits reactivity toward Mouse and Rat.

Quick Overview for SMPD1 antibody (AA 204-558) (ABIN7600575)

Target: SMPD1 (Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMPD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SMPD1 Antibody

Binding Specificity: AA 204-558

Purpose: Anti-Smpd1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Smpd1 Antibody (ABIN7600575). Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse Smpd1 recombinant protein (Position: D204-M558).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SMPD1

Target: SMPD1 (Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1))

Alternative Name: Smpd1

Background:

Synonyms: ATP-dependent RNA helicase DDX3X,3.6.4.13,DEAD box protein 3, X-chromosomal,DEAD box, X isoform,Helicase-like protein 2,HLP2,DDX3X,DBX, DDX3,

Tissue Specificity: Thymic medulla (at protein level). Prominently expressed in the small intestine, colon and appendix. Also found in thymus, spleen, lymph node and lung. The long form might be dominant in intestinal, and the short form in lymphoid tissues. Expressed by IL17 producing helper T-cells (Th17).

Background: Sphingomyelin phosphodiesterase 1 (SMPD1), also known as acid sphingomyelinase (ASM), is an enzyme that in humans is encoded by the SMPD1 gene. Enables acid sphingomyelin phosphodiesterase activity and zinc ion binding activity. Involved in ceramide biosynthetic process, positive regulation of apoptotic process, and response to ionizing radiation. Acts upstream of or within ceramide metabolic process, cholesterol metabolic process, and sphingomyelin catabolic process. Located in extracellular space. Is expressed in several structures, including alimentary system, integumental system, nervous system, sensory organ, and skeleton. Used to study Niemann-Pick disease. Human ortholog(s) of this gene implicated in Niemann-Pick disease, Niemann-Pick disease type A, and Niemann-Pick disease type B. Orthologous to human SMPD1 (sphingomyelin phosphodiesterase 1).

Molecular Weight: 70 kDa

Gene ID: 20597

UniProt: Q04519