C6orf150 antibody (AA 208-522)

Catalog No. ABIN7600586

This anti-C6orf150 antibody is a Rabbit Polyclonal antibody detecting C6orf150 in WB, ELISA, FACS, ICC and IF. Suitable for Human.

Quick Overview for C6orf150 antibody (AA 208-522) (ABIN7600586)

Target: C6orf150 (Chromosome 6 Open Reading Frame 150 (C6orf150))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C6orf150 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-C6orf150 Antibody

Binding Specificity: AA 208-522

Purpose: Anti-CGAS Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CGAS Antibody Picoband® (ABIN7600586). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CGAS recombinant protein (Position: L208-F522).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ablasser, A., Hemmerling, I., Schmid-Burgk, J. L., Behrendt, R., Roers, A., Hornung, V. TREX1 deficiency triggers cell-autonomous immunity in a cGAS-dependent manner. J. Immun. 192: 5993-5997, 2014. 2. Ablasser, A., Schmid-Burgk, J. L., Hemmerling, I., Horvath, G. L., Schmidt, T., Latz, E., Hornung, V. Cell intrinsic immunity spreads to bystander cells via the intercellular transfer of cGAMP. Nature 503: 530-534, 2013. 3. Andreeva, L., Hiller, B., Kostrewa, D., Lassig, C., de Oliveira Mann, C. C., Drexler, D. J., Maiser, A., Gaidt, M., Leonhardt, H., Hornung, V., Hopfner, K.-P. cGAS senses long and HMGB/TFAM-bound U-turn DNA by forming protein-DNA ladders Nature 549: 394-398, 2017.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for C6orf150

Target: C6orf150 (Chromosome 6 Open Reading Frame 150 (C6orf150))

Alternative Name: CGAS

Background:

Synonyms: Lymphocyte antigen 6A-2/6E-1,Ly-6A.2/Ly-6E.1,Stem cell antigen 1,SCA-1,T-cell-activating protein,TAP,Ly6a,Ly6,

Tissue Specificity: Widely expressed. .

Background: cGAS (cyclic GMP-AMP synthase), also known as MB21D1 (Mab-21 domain containing 1), h-cGAS or C6orf150, is a 522 amino acid cytoplasmic nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. cGAS is suggested to have antiviral activity by acting as a key cytosolic DNA sensor. cGAS binds to cytosolic DNA, which leads to cGAMP synthesis and activation of TMEM173, thereby trigger type-I interferon production. Expressed in monocytic cell line THP1, cGAS exists as two alternatively spliced isoforms and is encoded by a gene located on human chromosome 6q13.

Molecular Weight: 62 kDa

Gene ID: 115004

UniProt: Q8N884

Pathways: Activation of Innate immune Response