LY6E antibody (AA 21-101)

Catalog No. ABIN7600591

The Rabbit Polyclonal anti-LY6E antibody has been validated for ELISA, WB, IHC and FACS. It is suitable to detect LY6E in samples from Human.

Quick Overview for LY6E antibody (AA 21-101) (ABIN7600591)

Target: LY6E (Lymphocyte Antigen 6 Complex, Locus E (LY6E))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LY6E antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-LY6E Antibody

Binding Specificity: AA 21-101

Purpose: Anti-LY6E/SCA-2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-LY6E/SCA-2 Antibody Picoband® (ABIN7600591). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human LY6E/SCA-2 recombinant protein (Position: L21-S101).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Mao, M., Yu, M., Tong, J.-H., Ye, J., Zhu, J., Huang, Q.-H., Fu, G., Yu, L., Zhao, S.-Y., Waxman, S., Lanotte, M., Wang, Z.-Y., Tan, J.-Z., Chan, S.-J., Chen, Z. RIG-E, a human homolog of the murine Ly-6 family, is induced by retinoic acid during the differentiation of acute promyelocytic leukemia cell. Proc. Nat. Acad. Sci. 93: 5910-5914, 1996. 2. Shan, X., Bourdeau, A., Rhoton, A., Wells, D. E., Cohen, E. H., Landgraf, B. E., Palfree, R. G. E. Characterization and mapping to human chromosome 8q24.3 of Ly-6-related gene 9804 encoding an apparent homologue of mouse TSA-1. J. Immun. 160: 197-208, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for LY6E

Target: LY6E (Lymphocyte Antigen 6 Complex, Locus E (LY6E))

Alternative Name: LY6E

Background:

Synonyms: Mediator of RNA polymerase II transcription subunit 20, Mediator complex subunit 20, TRF-proximal protein homolog, hTRFP, MED20, TRFP,

Tissue Specificity: Pre-B-cells and B-cells but not terminally differentiated plasma cells.

Background: Lymphocyte antigen 6E is a protein that in humans is encoded by the LY6E gene. Increased expression of Ly6E is associated with poor survival outcome in multiple malignancies as determined by a survey of more than 130 published clinical studies of gene expression studies on cancer tissue samples and adjacent normal tissues. Ly6E is associated with drug resistance and tumor immune escape in breast cancer. Further research is required to validate Ly6E for translation research.

Molecular Weight: 14 kDa

Gene ID: 4061

UniProt: Q16553

Pathways: Hormone Transport