GLUD1 antibody (AA 210-558)

Catalog No. ABIN7600634

This anti-GLUD1 antibody is a Rabbit Polyclonal antibody detecting GLUD1 in WB, IHC, IP, ELISA, IF, ICC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for GLUD1 antibody (AA 210-558) (ABIN7600634)

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLUD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-GLUD1 Antibody

Binding Specificity: AA 210-558

Purpose: Anti-GLUD1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-GLUD1 Antibody Picoband® (ABIN7600634). Tested in ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GLUD1 recombinant protein (Position: A210-T558).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Smith TJ, Schmidt T, Fang J, Wu J, Siuzdak G, Stanley CA (May 2002). "The structure of apo human glutamate dehydrogenase details subunit communication and allostery". J. Mol. Biol. 318 (3): 765-77. 2. George A, Bell JE (December 1980). "Effects of adenosine 5'-diphosphate on bovine glutamate dehydrogenase: diethyl pyrocarbonate modification". Biochemistry. 19 (26): 6057-61. Glaser B, Blech I, Krakinovsky Y, Ekstein J, Gillis D, Mazor-Aronovitch K, Landau H, Abeliovich D (Oct 2011). "ABCC8 mutation allele frequency in the Ashkenazi Jewish population and risk of focal hyperinsulinemic hypoglycemia". Genetics in Medicine. 13 (10): 891-4.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for GLUD1

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Alternative Name: GLUD1

Background:

Synonyms: Double-strand-break repair protein rad21 homolog,hHR21,Nuclear matrix protein 1,NXP-1,SCC1 homolog,RAD21,HR21, KIAA0078, NXP1,

Tissue Specificity: Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues. .

Background: GLUD1 (glutamate dehydrogenase 1) is a mitochondrial matrix enzyme, one of the family of glutamate dehydrogenases that are ubiquitous in life, with a key role in nitrogen and glutamate (Glu) metabolism and energy homeostasis. This dehydrogenase is expressed at high levels in liver, brain, pancreas and kidney, but not in muscle. In the pancreatic cells, GLUD1 is thought to be involved in insulin secretion mechanisms. In nervous tissue, where glutamate is present in concentrations higher than in the other tissues, GLUD1 appears to function in both the synthesis and the catabolism of glutamate and perhaps in ammonia detoxification.

Molecular Weight: 55 kDa

Gene ID: 2746

UniProt: P00367

Pathways: Positive Regulation of Peptide Hormone Secretion, Warburg Effect