Nucleolar Protein 1 (NOL1) (AA 210-747) antibody

Catalog No. ABIN7600635

This Rabbit Polyclonal antibody specifically detects in WB, ELISA, IF, FACS and ICC. It exhibits reactivity toward Human.

Quick Overview for Nucleolar Protein 1 (NOL1) (AA 210-747) antibody (ABIN7600635)

Target: Nucleolar Protein 1 (NOL1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details

Binding Specificity: AA 210-747

Purpose: Anti-NOP2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NOP2 Antibody Picoband® (ABIN7600635). Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NOP2 recombinant protein (Position: L210-Q747).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Baens, M., Chaffanet, M., Aerssens, J., Cassiman, J.-J., Marynen, P. Assignment of the gene for the human proliferating cell nucleolar protein P120 (NOL1) to chromosome 12p13 by fluorescence in situ hybridization and polymerase chain reaction with somatic cell hybrids. Genomics 21: 296-297, 1994. 2. Fonagy, A., Swiderski, C., Wilson, A., Bolton, W., Kenyon, N., Freeman, J. W. Cell cycle regulated expression of nucleolar antigen P120 in normal and transformed human fibroblasts. J. Cell. Physiol. 154: 16-27, 1993. 3. Freeman, J. W., Busch, R. K., Gyorkey, F., Gyorkey, P., Ross, B. E., Busch, H. Identification and characterization of a human proliferation-associated nucleolar antigen with a molecular weight of 120,000 expressed in early G1 phase. Cancer Res. 48: 1244-1251, 1988.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details

Target: Nucleolar Protein 1 (NOL1)

Alternative Name: NOP2

Background:

Synonyms: NACHT, LRR and PYD domains-containing protein 2, Nucleotide-binding site protein 1, PYRIN domain and NACHT domain-containing protein 1, PYRIN-containing APAF1-like protein 2, NLRP2, NALP2, NBS1, PAN1, PYPAF2

Tissue Specificity: Expressed at high levels in lung, placenta and thymus and at lower levels in ovary, intestine and brain.

Background: Enables RNA binding activity. Involved in positive regulation of cell population proliferation, regulation of signal transduction by p53 class mediator, and ribosomal large subunit assembly. Located in nucleolus.

Molecular Weight: 120 kDa

Gene ID: 4839

UniProt: P46087