GPR50 antibody (AA 214-471)

Catalog No. ABIN7600650

This Rabbit Polyclonal antibody specifically detects GPR50 in WB and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for GPR50 antibody (AA 214-471) (ABIN7600650)

Target: GPR50 (G Protein-Coupled Receptor 50 (GPR50))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR50 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-GPR50 Antibody

Binding Specificity: AA 214-471

Purpose: Anti-GPR50 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-GPR50 Antibody Picoband® (ABIN7600650). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GPR50 recombinant protein (Position: K214-S471).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Gubitz, A. K., Reppert, S. M. Assignment of the melatonin-related receptor to human chromosome X (GPR50) and mouse chromosome X (Gpr50). Genomics 55: 248-251, 1999. 2. Levoye, A., Dam, J., Ayoub, M. A., Guillaume, J.-L., Couturier, C., Delagrange, P., Jockers, R. The orphan GPR50 receptor specifically inhibits MT1 melatonin receptor function through heterodimerization. EMBO J. 25: 3012-3023, 2006. 3. Reppert, S. M., Weaver, D. R., Ebisawa, T., Mahle, C. D., Kolakowski, L. F., Jr. Cloning of a melatonin-related receptor from human pituitary. FEBS Lett. 386: 219-224, 1996.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for GPR50

Target: GPR50 (G Protein-Coupled Receptor 50 (GPR50))

Alternative Name: GPR50

Background:

Synonyms: Protein SOX-15, Protein SOX-12, Protein SOX-20, SOX15, SOX12, SOX20, SOX26, SOX27

Tissue Specificity: Widely expressed in fetal and adult tissues examined, highest level found in fetal spinal cord and adult brain and testis.

Background: G protein-coupled receptor 50 is a protein which in humans is encoded by the GPR50 gene. This gene product belongs to the G-protein coupled receptor 1 family. Even though this protein shares similarity with the melatonin receptors, it does not bind melatonin, however, it inhibits melatonin receptor 1A function through heterodimerization. Polymorphic variants of this gene have been associated with bipolar affective disorder in women.

Molecular Weight: 67 kDa

Gene ID: 9248

UniProt: Q13585