SORBS1 antibody (AA 23-323)

Catalog No. ABIN7600747

The Rabbit Polyclonal anti-SORBS1 antibody has been validated for WB, ELISA, IHC and FACS. It is suitable to detect SORBS1 in samples from Human.

Quick Overview for SORBS1 antibody (AA 23-323) (ABIN7600747)

Target: SORBS1 (Sorbin and SH3 Domain Containing 1 (SORBS1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SORBS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-SORBS1 Antibody

Binding Specificity: AA 23-323

Purpose: Anti-SORBS1 Picoband® Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SORBS1 Picoband® Antibody (ABIN7600747). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SORBS1 recombinant protein (Position: Q23-R323).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Lin, W.-H., Chiu, K. C., Chang, H.-M., Lee, K.-C., Tai, T.-Y., Chuang, L.-M. Molecular scanning of the human sorbin and SH3-domain-containing-1 (SORBS1) gene: positive association of the T228A polymorphism with obesity and type 2 diabetes. Hum. Molec. Genet. 10: 1753-1760, 2001. 2. Mastick, C. C., Brady, M. J., Saltiel, A. R. Insulin stimulates the tyrosine phosphorylation of caveolin. J. Cell Biol. 129: 1523-1531, 1995. 3. Ribon, V., Printen, J. A., Hoffman, N. G., Kay, B. K., Saltiel, A. R. A novel, multifunctional c-Cbl binding protein in insulin receptor signaling in 3T3-L1 adipocytes. Molec. Cell. Biol. 18: 872-879, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SORBS1

Target: SORBS1 (Sorbin and SH3 Domain Containing 1 (SORBS1))

Alternative Name: SORBS1

Background:

Synonyms: Sorbin and SH3 domain-containing protein 1, Ponsin, SH3 domain protein 5, SH3P12, c-Cbl-associated protein, CAP, SORBS1, KIAA0894, KIAA1296, SH3D5

Tissue Specificity: Expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells.

Background: CAP/Ponsinprotein, also known as Sorbin and SH3 domain-containing protein 1 is a protein that in humans is encoded by the SORBS1 gene. It is mapped to 10q24.1. This gene encodes a CBL-associated protein which functions in the signaling and stimulation of insulin. Mutations in this gene may be associated with human disorders of insulin resistance. Alternative splicing results in multiple transcript variants.

Molecular Weight: 160 kDa

Gene ID: 10580

Pathways: Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process