MMS19 antibody (AA 239-998)

Catalog No. ABIN7600802

This anti-MMS19 antibody is a Rabbit Polyclonal antibody detecting MMS19 in WB, ELISA, IF, IHC, ICC and FACS. Suitable for Human and Mouse.

Quick Overview for MMS19 antibody (AA 239-998) (ABIN7600802)

Target: MMS19 (MMS19 Nucleotide Excision Repair Homolog (MMS19))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMS19 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-MMS19 Antibody

Binding Specificity: AA 239-998

Purpose: Anti-MMS19 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-MMS19 Antibody Picoband® (ABIN7600802). Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MMS19 recombinant protein (Position: E239-R998).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gari, K., Leon Ortiz, A. M., Borel, V., Flynn, H., Skehel, J. M., Boulton, S. J. MMS19 links cytoplasmic iron-sulfur cluster assembly to DNA metabolism. Science 337: 243-245, 2012. 2. Hatfield, M. D., Reis, A. M. C., Obeso, D., Cook, J. R., Thompson, D. M., Rao, M., Friedberg, E. C., Queimado, L. Identification of MMS19 domains with distinct functions in NER and transcription. DNA Repair 5: 914-924, 2006. 3. Ito, S., Tan, L. J., Andoh, D., Narita, T., Seki, M., Hirano, Y., Narita, K., Kuraoka, I., Hiraoka, Y., Tanaka, K. MMXD, a TFIIH-independent XPD-MMS19 protein complex involved in chromosome segregation. Molec. Cell 39: 632-640, 2010.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for MMS19

Target: MMS19 (MMS19 Nucleotide Excision Repair Homolog (MMS19))

Alternative Name: MMS19

Background:

Synonyms: ELAV-like protein 2, ELAV-like neuronal protein 1, Hu-antigen B, HuB, Nervous system-specific RNA-binding protein Hel-N1, ELAVL2, HUB

Tissue Specificity: Brain, neural-specific.

Background: MMS19 nucleotide excision repair protein homolog is a protein that in humans is encoded by the MMS19 gene. MMS19 (MMS19 nucleotide excision repair homolog), also known as MET18, is a 1,030 amino acid nuclear protein containing seven HEAT repeats that belongs to the MET18/MMS19 family. Via its interactions with TFIIH p80 and TFIIH p89 helicases, MMS19 plays a role in nucleotide excision repair (NER) and RNA polymerase II (Pol II) transcription. MMS19 may also function as a transcriptional coactivator of estrogen receptor. While ubiquitously expressed, highest levels of MMS19 have been found in testis. At least five distinct MMS19 protein isoforms exist, which are produced by alternative splicing events.

Molecular Weight: 113 kDa

Gene ID: 64210

UniProt: Q96T76

Pathways: Nuclear Hormone Receptor Binding