PRDM15 antibody (AA 24-1494)

Catalog No. ABIN7600810

The Rabbit Polyclonal anti-PRDM15 antibody has been validated for WB, ELISA, IHC, IF and FACS. It is suitable to detect PRDM15 in samples from Human.

Quick Overview for PRDM15 antibody (AA 24-1494) (ABIN7600810)

Target: PRDM15 (PR Domain Containing 15 (PRDM15))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRDM15 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-PRDM15 Antibody

Binding Specificity: AA 24-1494

Purpose: Anti-PRDM15 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-PRDM15 Antibody Picoband® (ABIN7600810). Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PRDM15 recombinant protein (Position: D24-Q1494).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Mzoughi, S., Zhang, J., Hequet, D., Teo, S. X., Fang, H., Xing, Q. R., Bezzi, M., Seah, M. K. Y., Ong, S. L. M., Shin, E. M., Wollmann, H., Wong, E. S. M., Al-Haddawi, M., Stewart, C. L., Tergaonkar, V., Loh, Y.-H., Dunn, N. R., Messerschmidt, D. M., Guccione, E. PRDM15 safeguards naive pluripotency by transcriptionally regulating WNT and MAP-ERK signaling. Nature Genet. 49: 1354-1363, 2017. 2. Shibuya, K., Kudoh, J., Okui, M., Shimizu, N. Identification of a novel zinc finger protein gene (ZNF298) in the GAP2 of human chromosome 21q. Biochem. Biophys. Res. Commun. 332: 557-568, 2005.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PRDM15

Target: PRDM15 (PR Domain Containing 15 (PRDM15))

Alternative Name: PRDM15

Background:

Synonyms: RNA-binding protein 47,RNA-binding motif protein 47,RBM47,

Tissue Specificity: Abundantly expressed in tonsil, lymph node, and trachea, strong expression in prostate, lower expression in thyroid, stomach, and colon. .

Background: PR/SET domain 15 is a protein that in humans is encoded by the PRDM15 gene. Predicted to enable DNA-binding transcription activator activity, RNA polymerase II-specific, RNA polymerase II cis-regulatory region sequence-specific DNA binding activity, and promoter-specific chromatin binding activity. Predicted to be involved in positive regulation of transcription by RNA polymerase II, regulation of signal transduction, and regulation of stem cell division. Located in nuclear body.

Molecular Weight: 169 kDa

Gene ID: 63977

UniProt: P57071