POU6F2 antibody (AA 24-446)

Catalog No. ABIN7600834

The Rabbit Polyclonal anti-POU6F2 antibody has been validated for WB and ELISA. It is suitable to detect POU6F2 in samples from Human, Mouse and Rat.

Quick Overview for POU6F2 antibody (AA 24-446) (ABIN7600834)

Target: POU6F2 (POU Class 6 Homeobox 2 (POU6F2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POU6F2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-POU6F2 Antibody

Binding Specificity: AA 24-446

Purpose: Anti-POU6F2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-POU6F2 Antibody Picoband® (ABIN7600834). Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human POU6F2 recombinant protein (Position: E24-Q446). Human POU6F2 shares 89.7% amino acid (aa) sequence identity with mouse POU6F2.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/mL
1. Gross, M. B. Personal Communication. Baltimore, Md. 4/5/2019. 2. Perotti, D., De Vecchi, G., Testi, M. A., Lualdi, E., Modena, P., Mondini, P., Ravagnani, F., Collini, P., Di Renzo, F., Spreafico, F., Terenziani, M., Sozzi, G., Fossati-Bellani, F., Radice, P. Germline mutations of the POU6F2 gene in Wilms tumors with loss of heterozygosity on chromosome 7p14. Hum. Mutat. 24: 400-407, 2004. 3. Perotti, D., Testi, M. A., Mondini, P., Pilotti, S., Green, E. D., Pession, A., Sozzi, G., Pierotti, M. A., Fossati Bellani, F., Radice, P. Refinement within single yeast artificial chromosome clones of a minimal region commonly deleted on the short arm of chromosome 7 in Wilms tumours. Genes Chromosomes Cancer 31: 42-47, 2001.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for POU6F2

Target: POU6F2 (POU Class 6 Homeobox 2 (POU6F2))

Alternative Name: POU6F2

Background:

Synonyms: POU6F2, RPF1, POU domain, class 6, transcription factor 2, Retina-derived POU domain factor 1, RPF-1

Background: This gene encodes a member of the POU protein family characterized by the presence of a bipartite DNA binding domain, consisting of a POU-specific domain and a homeodomain, separated by a variable polylinker. The DNA binding domain may bind to DNA as monomers or as homo- and/or heterodimers, in a sequence-specific manner. The POU family members are transcriptional regulators, many of which are known to control cell type-specific differentiation pathways. This gene is a tumor suppressor involved in Wilms tumor (WT) predisposition. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Molecular Weight: 73 kDa

Gene ID: 11281

UniProt: P78424