TMEM129 antibody (AA 25-362)

Catalog No. ABIN7600907

Product Details anti-TMEM129 Antibody

Target: TMEM129 (Transmembrane Protein 129 (TMEM129))

Binding Specificity: AA 25-362

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Purpose: Anti-TMEM129 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TM Antibody Picoband® (ABIN7600907). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TMEM129 recombinant protein (Position: E25-R362).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hartz, P. A. Personal Communication. Baltimore, Md. 9/2/2014. 2. van den Boomen, D. J. H., Timms, R. T., Grice, G. L., Stagg, H. R., Skodt, K., Dougan, G., Nathan, J. A., Lehner, P. J. TMEM129 is a Derlin-1 associated ERAD E3 ligase essential for virus-induced degradation of MHC-I. Proc. Nat. Acad. Sci. 111: 11425-11430, 2014.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TMEM129

Target: TMEM129 (Transmembrane Protein 129 (TMEM129))

Alternative Name: TMEM129 (TMEM129 Products)

Background:

Synonyms: Protein eva-1 homolog A, Protein FAM176A, Transmembrane protein 166, EVA1A, FAM176A, TMEM166, SP24

Tissue Specificity: Expressed in lung, kidney, liver, pancreas, placenta, but not in heart and skeletal muscle.

Background: Enables ubiquitin protein ligase activity. Involved in protein polyubiquitination, retrograde protein transport, ER to cytosol, and ubiquitin-dependent ERAD pathway. Located in endoplasmic reticulum.

Molecular Weight: 50 kDa

Gene ID: 92305