Inhibin alpha antibody (AA 254-366)

Catalog No. ABIN7600932

This Rabbit Polyclonal antibody specifically detects Inhibin alpha in WB, IHC, ELISA, ICC, IF and FACS. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for Inhibin alpha antibody (AA 254-366) (ABIN7600932)

Target: Inhibin alpha (INHA) (Inhibin, alpha (INHA))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Inhibin alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Inhibin alpha Antibody

Binding Specificity: AA 254-366

Purpose: Anti-Inhibin alpha/INHA Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Inhibin alpha/INHA Antibody Picoband® (ABIN7600932). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Inhibin alpha/INHA recombinant protein (Position: E254-I366).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Arunachalam, B., Phan, U. T., Geuze, H. J., Cresswell, P. Enzymatic reduction of disulfide bonds in lysosomes: characterization of a gamma-interferon-inducible lysosomal thiol reductase (GILT). Proc. Nat. Acad. Sci. 97: 745-750, 2000. 2. Maric, M., Arunachalam, B., Phan, U. T., Dong, C., Garrett, W. S., Cannon, K. S., Alfonso, C., Karlsson, L., Flavell, R. A., Cressell, P. Defective antigen processing in GILT-free mice. Science 294: 1361-1365, 2001. 3. Phan, U. T., Arunachalam, B., Cresswell, P. Gamma-interferon-inducible lysosomal thiol reductase (GILT): maturation, activity, and mechanism of action. J. Biol. Chem. 275: 25907-25914, 2000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Inhibin alpha

Target: Inhibin alpha (INHA) (Inhibin, alpha (INHA))

Alternative Name: INHA

Background:

Synonyms: Bromodomain-containing protein 2, O27.1.1, Really interesting new gene 3 protein, BRD2, KIAA9001, RING3

Tissue Specificity: Expressed in thymus, spleen, bone marrow, kidney, brain and testis, but hardly in all other tissues or in whole embryos during development.

Background: Inhibin, alpha, also known as INHA, is a protein which in humans is encoded by the INHA gene. This gene encodes a member of the TGF-beta (transforming growth factor-beta) superfamily of proteins. The encoded preproprotein is proteolytically processed to generate multiple peptide products, including the alpha subunit of the inhibin A and B protein complexes. These complexes negatively regulate follicle stimulating hormone secretion from the pituitary gland. Inhibins have also been implicated in regulating numerous cellular processes including cell proliferation, apoptosis, immune response and hormone secretion. Mutations in this gene may be associated with male infertility and premature ovarian failure in female human patients.

Molecular Weight: 45 kDa

Gene ID: 3623

UniProt: P05111

Pathways: Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion