Rhotekin antibody (AA 255-554)

Catalog No. ABIN7600934

This anti-Rhotekin antibody is a Rabbit Polyclonal antibody detecting Rhotekin in WB, ELISA and FACS. Suitable for Human.

Quick Overview for Rhotekin antibody (AA 255-554) (ABIN7600934)

Target: Rhotekin (RTKN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Rhotekin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-Rhotekin Antibody

Binding Specificity: AA 255-554

Purpose: Anti-RTKN Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RTKN Antibody Picoband® (ABIN7600934). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RTKN recombinant protein (Position: R255-Q554).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Jang, W., Weber, J. S., Harkins, E. B., Meisler, M. H. Localization of the rhotekin gene RTKN on the physical maps of mouse chromosome 6 and human chromosome 2p13 and exclusion as a candidate for mnd2 and LGMD2B. Genomics 40: 506-507, 1997. 2. Reid, T., Furuyashiki, T., Ishizaki, T., Watanabe, G., Watanabe, N., Fujisawa, K., Morii, N., Madaule, P., Narumiya, S. Rhotekin, a new putative target for Rho bearing homology to a serine/threonine kinase, PKN, and rhophilin in the Rho-binding domain. J. Biol. Chem. 271: 13556-13560, 1996. 3. Sudo, K., Ito, H., Iwamoto, I., Morishita, R., Asano, T., Nagata, K. Identification of a cell polarity-related protein, Lin-7B, as a binding partner for a Rho effector, rhotekin, and their possible interaction in neurons. Neurosci. Res. 56: 347-355, 2006.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Rhotekin

Target: Rhotekin (RTKN)

Alternative Name: RTKN

Background:

Synonyms: C-C motif chemokine 16, Chemokine CC-4, HCC-4, Chemokine LEC, IL-10-inducible chemokine, LCC-1, Liver-expressed chemokine, Lymphocyte and monocyte chemoattractant, LMC, Monotactin-1, MTN-1, NCC-4, Small-inducible cytokine A16, CCL16, ILINCK, NCC4, SCYA16

Tissue Specificity: Mainly expressed in liver, also found in spleen and thymus. Highly expressed in LPS- and IFN-gamma- activated monocytes, weakly in some lymphocytes, including natural killer cells, gamma-delta T-cells, and some T-cell clones.

Background: Rhotekin is a protein that in humans is encoded by the RTKN gene. This gene encodes a scaffold protein that interacts with GTP-bound Rho proteins. Binding of this protein inhibits the GTPase activity of Rho proteins. This protein may interfere with the conversion of active, GTP-bound Rho to the inactive GDP-bound form by RhoGAP. Rho proteins regulate many important cellular processes, including cytokinesis, transcription, smooth muscle contraction, cell growth and transformation. Dysregulation of the Rho signal transduction pathway has been implicated in many forms of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 70 kDa

Gene ID: 6242