PYROXD1 antibody (AA 26-453)

Catalog No. ABIN7600963

The Rabbit Polyclonal anti-PYROXD1 antibody has been validated for WB, ELISA, FACS, IF and ICC. It is suitable to detect PYROXD1 in samples from Human, Rat and Mouse.

Quick Overview for PYROXD1 antibody (AA 26-453) (ABIN7600963)

Target: PYROXD1 (Pyridine Nucleotide-Disulphide Oxidoreductase Domain 1 (PYROXD1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PYROXD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-PYROXD1 Antibody

Binding Specificity: AA 26-453

Purpose: Anti-PYROXD1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PYROXD1 Antibody Picoband® (ABIN7600963). Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PYROXD1 recombinant protein (Position: A26-N453).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. O'Grady, G. L., Best, H. A., Sztal, T. E., Schartner, V., Sanjuan-Vazquez, M., Donkervoort, S., Neto, O. A., Sutton, R. B., Ilkovski, B., Romero, N. B., Stojkovic, T., Dastgir, J., and 30 others. Variants in the oxidoreductase PYROXD1 cause early-onset myopathy with internalized nuclei and myofibrillar disorganization. Am. J. Hum. Genet. 99: 1086-1105, 2016. 2. Zhang, J.-L., Zhao, W.-G., Wu, K.-L., Wang, K., Zhang, X., Gu, C.-F., Li, Y., Zhu, Y., Wu, J.-G. Human hepatitis B virus X protein promotes cell proliferation and inhibits cell apoptosis through interacting with a serine protease Hepsin. Arch. Virol. 150: 721-741, 2005.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PYROXD1

Target: PYROXD1 (Pyridine Nucleotide-Disulphide Oxidoreductase Domain 1 (PYROXD1))

Alternative Name: PYROXD1

Background:

Synonyms: Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 1, PYROXD1

Tissue Specificity: Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

Background: Pyridine nucleotide-disulphide oxidoreductase domain 1 is a protein that in humans is encoded by the PYROXD1 gene. It is mapped to 12p12.1. This gene encodes a nuclear-cytoplasmic pyridine nucleotide-disulphide reductase (PNDR). PNDRs are flavoproteins that catalyze the pyridine nucleotide-dependent reduction of thiol residues in other proteins. The encoded protein belongs to the class I pyridine nucleotide-disulphide oxidoreductase family but lacks the C-terminal dimerization domain found in other family members and instead has a C-terminal nitrile reductase domain. It localizes to the nucleus and to striated sarcomeric compartments. Naturally occurring mutations in this gene cause early-onset myopathy with internalized nuclei and myofibrillar disorganization. A pseudogene of this gene has been defined on chromosome 11.

Molecular Weight: 65 kDa

Gene ID: 79912