NAD-ME antibody (AA 26-584)

Catalog No. ABIN7600967

This anti-NAD-ME antibody is a Rabbit Polyclonal antibody detecting NAD-ME in WB, ELISA, IHC, IF, ICC and FACS. Suitable for Human, Mouse, Rat and Monkey.

Quick Overview for NAD-ME antibody (AA 26-584) (ABIN7600967)

Target: NAD-ME (NAD Dependent Malate Dehydrogenase (NAD-ME))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAD-ME antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-NAD-ME Antibody

Binding Specificity: AA 26-584

Purpose: Anti-ME2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ME2 Antibody Picoband® (ABIN7600967). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ME2 recombinant protein (Position: K26-E584).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Monkey, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Burchell, A., Crosby, A., Cohen, P. T. W. Human mitochondrial malic enzyme variants: properties of the different polymorphic forms. Ann. Hum. Genet. 41: 1-7, 1977. 2. Champion, M. J., Brown, J. A., Shows, T. B. Assignment of cytoplasmic alpha-mannosidase (MAN-A) and confirmation of mitochondrial isocitrate dehydrogenase (IDH-M) to the q11-qter region of chromosome 15 in man. Cytogenet. Cell Genet. 22: 498-502, 1978. 3. Cohen, P. T. W., Omenn, G. S. Genetic variation of the cytoplasmic and mitochondrial malic enzymes in the monkey: Macaca nemestrina. Biochem. Genet. 7: 289-301, 1972.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for NAD-ME

Target: NAD-ME (NAD Dependent Malate Dehydrogenase (NAD-ME))

Alternative Name: ME2

Background:

Synonyms: NAD-dependent malic enzyme, mitochondrial, NAD-ME, Malic enzyme 2, ME2

Tissue Specificity: Ubiquitous. Highly expressed in testis.

Background: NAD-dependent malic enzyme, mitochondrial is a protein that in humans is encoded by the ME2 gene. It is mapped to 18q21.2. This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, that catalyzes the oxidative decarboxylation of malate to pyruvate. It had previously been weakly linked to a syndrome known as Friedreich ataxia that has since been shown to be the result of mutation in a completely different gene. Certain single-nucleotide polymorphism haplotypes of this gene have been shown to increase the risk for idiopathic generalized epilepsy. Alternatively spliced transcript variants encoding different isoforms found for this gene.

Molecular Weight: 65 kDa

Gene ID: 4200

UniProt: P23368

Pathways: Production of Molecular Mediator of Immune Response