LGR5 antibody (AA 26-907)

Catalog No. ABIN7600974

This Rabbit Polyclonal antibody specifically detects LGR5 in WB, FACS, ELISA and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for LGR5 antibody (AA 26-907) (ABIN7600974)

Target: LGR5 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LGR5 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC)

Product Details anti-LGR5 Antibody

Binding Specificity: AA 26-907

Purpose: Anti-LGR5 Antibody

Characteristics: Anti-LGR5 Antibody (ABIN7600974). Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human LGR5 recombinant protein (Position: R26-L907). Human LGR5 shares 87% and 87.1% amino acid (aa) sequence identity with mouse and rat LGR5, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry, 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for LGR5

Target: LGR5 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5))

Alternative Name: LGR5

Background: Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5) also known as G-protein coupled receptor 49 (GPR49) or G-protein coupled receptor 67 (GPR67) is a protein that in humans is encoded by the LGR5 gene. The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 130 kDa

Gene ID: 8549

UniProt: O75473

Pathways: WNT Signaling