FAM59A antibody (AA 277-753)

Catalog No. ABIN7601055

This Rabbit Polyclonal antibody specifically detects FAM59A in ELISA, WB, IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for FAM59A antibody (AA 277-753) (ABIN7601055)

Target: FAM59A (Family with Sequence Similarity 59, Member A (FAM59A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAM59A antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-FAM59A Antibody

Binding Specificity: AA 277-753

Purpose: Anti-GAREM1 Antibody Picoband®

Characteristics: Anti-GAREM1 Antibody Picoband® (ABIN7601055). Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GAREM1 recombinant protein (Position: Q277-E753). Human GAREM1 shares 86.8% amino acid (aa) sequence identity with mouse GAREM1.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry, 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Nishino, T., Matsunaga, R., Konishi, H. Functional relationship between CABIT, SAM and 14-3-3 binding domains of GAREM1 that play a role in its subcellular localization. Biochem. Biophys. Res. Commun. 464: 616-621, 2015. 2. Taniguchi, T., Tanaka, S., Ishii, A., Watanabe, M., Fujitani, N., Sugeo, A., Gotoh, S., Ohta, T., Hiyoshi, M., Matsuzaki, H., Sakai, N., Konishi, H. A brain-specific Grb2-associated regulator of extracellular signal-regulated kinase (Erk)mitogen-activated protein kinase (MAPK) (GAREM) subtype, GAREM2, contributes to neurite outgrowth of neuroblastoma cells by regulating Erk signaling. J. Biol. Chem. 288: 29934-29942, 2013. 3. Tashiro, K., Tsunematsu, T., Okubo, H., Ohta, T., Sano, E., Yamauchi, E., Taniguchi, H., Konishi, H. GAREM, a novel adaptor protein for growth factor receptor-bound protein 2, contributes to cellular transformation through activation of extracellular signal-regulated kinase signaling. J. Biol. Chem. 284: 20206-20214, 2009.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for FAM59A

Target: FAM59A (Family with Sequence Similarity 59, Member A (FAM59A))

Alternative Name: GAREM1

Background: This gene encodes an adaptor protein which functions in the epidermal growth factor (EGF) receptor-mediated signaling pathway. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 97 kDa

Gene ID: 64762

UniProt: Q9H706

Pathways: EGFR Signaling Pathway