SPAG16 antibody (AA 28-619)

Catalog No. ABIN7601085

This Rabbit Polyclonal antibody specifically detects SPAG16 in WB, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SPAG16 antibody (AA 28-619) (ABIN7601085)

Target: SPAG16 (Sperm Associated Antigen 16 (SPAG16))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPAG16 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-SPAG16 Antibody

Binding Specificity: AA 28-619

Purpose: Anti-SPAG16 Antibody Picoband®

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Anti-SPAG16 Antibody Picoband® (ABIN7599569). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SPAG16 recombinant protein (Position: D28-F619). Human SPAG16 shares 75.1% amino acid (aa) sequence identity with mouse SPAG16.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Pennarun, G., Bridoux, A.-M., Escudier, E., Dastot-Le Moal, F., Cacheux, V., Amselem, S., Duriez, B. Isolation and expression of the human hPF20 gene orthologous to Chlamydomonas pf20: evaluation as a candidate for axonemal defects of respiratory cilia and sperm flagella. Am. J. Resp. Cell Molec. Biol. 26: 362-370, 2002. 2. Wilson, C. W., Nguyen, C. T., Chen, M.-H., Yang, J.-H., Gacayan, R., Huang, J., Chen, J.-N., Chuang, P.-T. Fused has evolved divergent roles in vertebrate hedgehog signalling and motile ciliogenesis. Nature 459: 98-102, 2009. 3. Zhang, Z., Kostetskii, I., Moss, S. B., Jones, B. H., Ho, C., Wang, H., Kishida, T., Gerton, G. L., Radice, G. L., Strauss, J. F., III. Haploinsufficiency for the murine orthologue of Chlamydomonas PF20 disrupts spermatogenesis. Proc. Nat. Acad. Sci. 101: 12946-12951, 2004.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SPAG16

Target: SPAG16 (Sperm Associated Antigen 16 (SPAG16))

Alternative Name: SPAG16

Background:

Synonyms: 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70 kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody

Tissue Specificity: Expressed in all tissues.

Background: Cilia and flagella are comprised of a microtubular backbone, the axoneme, which is organized by the basal body and surrounded by plasma membrane. SPAG16 encodes 2 major proteins that associate with the axoneme of sperm tail and the nucleus of postmeiotic germ cells, respectively

Molecular Weight: 71 kDa

Gene ID: 79582

UniProt: Q8N0X2