ALDH1L1 antibody (AA 282-424)

Catalog No. ABIN7601093

This anti-ALDH1L1 antibody is a Rabbit Polyclonal antibody detecting ALDH1L1 in WB, IHC, ELISA, IF, FACS and ICC. Suitable for Human, Mouse, Rat and Monkey.

Quick Overview for ALDH1L1 antibody (AA 282-424) (ABIN7601093)

Target: ALDH1L1 (Aldehyde Dehydrogenase 1 Family, Member L1 (ALDH1L1))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALDH1L1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-ALDH1L1 Antibody

Binding Specificity: AA 282-424

Purpose: Anti-ALDH1L1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ALDH1L1 Antibody Picoband® (ABIN7601093). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ALDH1L1 recombinant protein (Position: N282-L424).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Champion, K. M., Cook, R. J., Tollaksen, S. L., Giometti, C. S. Identification of a heritable deficiency of the folate-dependent enzyme 10-formyltetrahydrofolate dehydrogenase in mice. Proc. Nat. Acad. Sci. 91: 11338-11342, 1994. 2. Gross, M. B. Personal Communication. Baltimore, Md. 11/2/2010. 3. Hong, M., Lee, Y., Kim, J. W., Lim, J. S., Chang, S. Y., Lee, K. S., Paik, S. G., Choe, I. S. Isolation and characterization of cDNA clone for human liver 10-formyltetrahydrofolate dehydrogenase. Biochem. Molec. Biol. Int. 47: 407-415, 1999.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ALDH1L1

Target: ALDH1L1 (Aldehyde Dehydrogenase 1 Family, Member L1 (ALDH1L1))

Alternative Name: ALDH1L1

Background:

Synonyms: Type I iodothyronine deiodinase, 5DI, DIOI, Type 1 DI, Type-I 5'-deiodinase, DIO1, ITDI1, TXDI1

Tissue Specificity: Widely expressed in adult (at protein level) and fetal tissues.

Background: 10-formyltetrahydrofolate dehydrogenase is an enzyme that in humans is encoded by the ALDH1L1 gene. The protein encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, NADP, and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family and is responsible for formate oxidation in vivo. Deficiencies in this gene can result in an accumulation of formate and subsequent methanol poisoning.

Molecular Weight: 97 kDa

Gene ID: 10840

UniProt: O75891