HNRNPH3 antibody (AA 29-268)

Catalog No. ABIN7601121

This Rabbit Polyclonal antibody specifically detects HNRNPH3 in WB, ELISA, IHC, IF, ICC and FACS. It exhibits reactivity toward Human.

Quick Overview for HNRNPH3 antibody (AA 29-268) (ABIN7601121)

Target: HNRNPH3 (Heterogeneous Nuclear Ribonucleoprotein H3 (2H9) (HNRNPH3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNRNPH3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-HNRNPH3 Antibody

Binding Specificity: AA 29-268

Purpose: Anti-HNRNPH3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-HNRNPH3 Antibody Picoband® (ABIN7601121). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HNRNPH3 recombinant protein (Position: K29-N268).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 1-2 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -,1. Mahe, D., Mahl, P., Gattoni, R., Fischer, N., Mattei, M.-G., Stevenin, J., Fuchs, J.-P. Cloning of human 2H9 heterogeneous nuclear ribonucleoproteins. J. Biol. Chem. 272: 1827-1836, 1997.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for HNRNPH3

Target: HNRNPH3 (Heterogeneous Nuclear Ribonucleoprotein H3 (2H9) (HNRNPH3))

Alternative Name: HNRNPH3

Background:

Synonyms: Interleukin-17B, IL-17B, Cytokine CX1, Cytokine-like protein ZCYTO7, Neuronal interleukin-17-related factor, Il17b, Nirf, Zcyto7

Tissue Specificity: Expressed in adult pancreas, small intestine, stomach, spinal cord and testis. Less pronounced expression in prostate, colon mucosal lining, and ovary.

Background: Heterogeneous nuclear ribonucleoprotein H3 is a protein that in humans is encoded by the HNRNPH3 gene. This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It is localized in nuclear bodies of the nucleus. This protein is involved in the splicing process and it also participates in early heat shock-induced splicing arrest by transiently leaving the hnRNP complexes. Several alternatively spliced transcript variants have been noted for this gene, however, not all are fully characterized.

Molecular Weight: 35 kDa, 37 kDa

Gene ID: 3189

UniProt: P31942