MFAP3L antibody (AA 29-409)

Catalog No. ABIN7601132

This anti-MFAP3L antibody is a Rabbit Polyclonal antibody detecting MFAP3L in ELISA, WB, IF and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for MFAP3L antibody (AA 29-409) (ABIN7601132)

Target: MFAP3L (Microfibrillar-Associated Protein 3-Like (MFAP3L))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MFAP3L antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-MFAP3L Antibody

Binding Specificity: AA 29-409

Purpose: Anti-MFAP3L Antibody Picoband®

Characteristics: Anti-MFAP3L Antibody Picoband® (ABIN7601132). Tested in WB, ICC/IF, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MFAP3L recombinant protein (Position: K29-V409). Human MFAP3L shares 90.6% amino acid (aa) sequence identity with both mouse and rat MFAP3L.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ishikawa, K., Nagase, T., Suyama, M., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res. 5: 169-176, 1998. 2. Lou, X., Kang, B., Zhang, J., Hao, C., Tian, X., Li, W., Xu, N., Lu, Y., Liu, S. MFAP3L activation promotes colorectal cancer cell invasion and metastasis. Biochim. Biophys. Acta 1842: 1423-1432, 2014.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for MFAP3L

Target: MFAP3L (Microfibrillar-Associated Protein 3-Like (MFAP3L))

Alternative Name: MFAP3L

Background: Microbibrillar-Associated Protein 3-Like (MFAP3L), also known as NYD-sp9, is part of the microfibrillar-associated protein family (MFAPs). MFAPs are non-fibrillin, extracellular matrix glycoproteins that interact with fibrillin and were originally characterized in microfibrillar assembly. In humans, there several subfamily members with varying amino acid (aa) sequence homology and functions . Among the family, MFAP2 and MFAP5 are more closely related and while MFAP1, 3 and 4 share no structural or sequence homology with MFAP2, MFAP5 or with each other. Human MFAP3L shows 71 % amino acid (aa) sequence homology to MFAP3, but not other MFAPs. Mature, human MFAP3L consists of an extracellular domain (ECD) containing N-linked glycosylation sites, a transmembrane domain, and a cytoplasmic domain with a conserved SH2 motif. The ECD of human MFAP3L shares 89 % and 90 % aa sequence identity with mouse and rat MFAP3L, respectively. MFAPs have the unique ability to interact with TGF-beta family growth factors, Notch and Notch ligands and multiple elastic fiber proteins, in addition to interacting with fibrillin. MFAPs are expressed in a wide variety of tissues and, along with microfibril assembly, they play roles in the regulation of tissue homeostasis, cell survival, and tumor progression. MFAP3L is often located within colorectal cancer (CRC) cells, which metastasize by activation of the nuclear ERK pathway via MFAP3L phosphorylation. Regulation of this MFAP3L activity could have pharmaceutical effects on CRC tumor progression.

Molecular Weight: 45 kDa

Gene ID: 9848

UniProt: O75121