CTHRC1 antibody (AA 31-243)

Catalog No. ABIN7601256

This Rabbit Polyclonal antibody specifically detects CTHRC1 in WB, ELISA, IHC, FACS, ICC and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for CTHRC1 antibody (AA 31-243) (ABIN7601256)

Target: CTHRC1 (Collagen Triple Helix Repeat Containing 1 (CTHRC1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CTHRC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-CTHRC1 Antibody

Binding Specificity: AA 31-243

Purpose: Anti-CTHRC1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CTHRC1 Antibody Picoband® (ABIN7601256). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CTHRC1 recombinant protein (Position: S31-K243).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 4 μg/mL, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Orloff, M., Peterson, C., He, X., Ganapathi, S., Heald, B., Yang, Y., Bebek, G., Romigh, T., Song, J. H., Wu, W., David, S., Cheng, Y., Meltzer, S. J., Eng, C. Germline mutations in MSR1, ASCC1, and CTHRC1 in patients with Barrett esophagus and esophageal adenocarcinoma. JAMA 306: 410-419, 2011. 2. Pyagay, P., Heroult, M., Wang, Q., Lehnert, W., Belden, J., Liaw, L., Friesel, R. E., Lindner, V. Collagen triple helix repeat containing 1, a novel secreted protein in injured and disease arteries, inhibits collagen expression and promotes cell migration. Circ. Res. 96: 261-268, 2005. 3. Yamamoto, S., Nishimura, O., Misaki, K., Nishita, M., Minami, Y., Yonemura, S., Tarui, H., Sasaki, H. Cthrc1 selectively activates the planar cell polarity pathway of Wnt signaling by stabilizing the Wnt-receptor complex. Dev. Cell 15: 23-36, 2008.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CTHRC1

Target: CTHRC1 (Collagen Triple Helix Repeat Containing 1 (CTHRC1))

Alternative Name: CTHRC1

Background:

Synonyms: Visual system homeobox 2,Ceh-10 homeodomain-containing homolog,Homeobox protein CHX10,VSX2,CHX10, HOX10,

Tissue Specificity: Abundantly expressed in retinal neuroblasts during eye development and in the inner nuclear layer of the adult retina. Within this layer, expression is stronger in the outer margin where bipolar cells predominate.

Background: This locus encodes a protein that may play a role in the cellular response to arterial injury through involvement in vascular remodeling. Mutations at this locus have been associated with Barrett esophagus and esophageal adenocarcinoma. Alternatively spliced transcript variants have been described. The International Radiation Hybrid Mapping Consortium mapped the CTHRC1 gene to chromosome.

Molecular Weight: 26 kDa

Gene ID: 115908

Pathways: Tube Formation