SIGLEC12 antibody (AA 31-287)

Catalog No. ABIN7601259

This Rabbit Polyclonal antibody specifically detects SIGLEC12 in WB, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SIGLEC12 antibody (AA 31-287) (ABIN7601259)

Target: SIGLEC12 (Sialic Acid Binding Ig-Like Lectin 12 (SIGLEC12))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIGLEC12 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-SIGLEC12 Antibody

Binding Specificity: AA 31-287

Purpose: Anti-SIGLEC12 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SIGLEC12 Antibody Picoband® (ABIN7601259). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SIGLEC12 recombinant protein (Position: S31-H287).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Angata, T., Varki, N. M., Varki, A. A second uniquely human mutation affecting sialic acid biology. J. Biol. Chem. 276: 40282-40287, 2001. 2. Foussias, G., Taylor, S. M., Yousef, G. M., Tropak, M. B., Ordon, M. H., Diamandis, E. P. Cloning and molecular characterization of two splice variants of a new putative member of the Siglec-3-like subgroup of Siglecs. Biochem. Biophys. Res. Commun. 284: 887-899, 2001. 3. Mitra, N., Banda, K., Altheide, T. K., Schaffer, L., Johnson-Pais, T. L., Beuten, J., Leach, R. J., Angata, T., Varki, N., Varki, A. SIGLEC12, a human-specific segregating (pseudo)gene, encodes a signaling molecule expressed in prostate carcinomas. J. Biol. Chem. 286: 23003-23011, 2011.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SIGLEC12

Target: SIGLEC12 (Sialic Acid Binding Ig-Like Lectin 12 (SIGLEC12))

Alternative Name: SIGLEC12

Background:

Synonyms: Neutrophil defensin 1,Defensin, alpha 1,HNP-1,HP-1,HP1,HP 1-56,Neutrophil defensin 2,HNP-2,HP-2,HP2,DEFA1,DEF1, DEFA2, MRS,DEFA1B,

Tissue Specificity: Expressed in immature but not mature T-cells. Also found in CD34+ cells from peripheral blood, CD34+ precursors from umbilical cord blood and adult bone marrow.

Background: Sialic acid-binding Ig-like lectin 12, or Siglec-XII, is a protein that in humans, is encoded by the SIGLEC12 gene. Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. This gene encodes a member of the SIGLEC3-like subfamily of SIGLECs. Members of this subfamily are characterized by an extracellular V-set immunoglobulin-like domain followed by two C2-set immunoglobulin-like domains, and the cytoplasmic tyrosine-based motifs ITIM and SLAM-like. The encoded protein, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containing protein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negative regulation of macrophage signaling by functioning as an inhibitory receptor. This gene is located in a cluster with other SIGLEC3-like genes on 19q13.4. Alternative splicing results in multiple transcript variants.

Molecular Weight: 70 kDa

Gene ID: 89858