ACAT2 antibody (AA 31-397)

Catalog No. ABIN7601268

This Rabbit Polyclonal antibody specifically detects ACAT2 in WB, FACS and ELISA. It exhibits reactivity toward Human and Mouse.

Quick Overview for ACAT2 antibody (AA 31-397) (ABIN7601268)

Target: ACAT2 (Acetyl-CoA Acetyltransferase 2 (ACAT2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACAT2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Product Details anti-ACAT2 Antibody

Binding Specificity: AA 31-397

Purpose: Anti-ACAT2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ACAT2 Antibody Picoband® (ABIN7601268). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ACAT2 recombinant protein (Position: Q31-E397).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ashworth, A. Two acetyl-CoA acetyltransferase genes located in the t-complex region of mouse chromosome 17 partially overlap the Tcp-1 and Tcp-1x genes. Genomics 18: 195-198, 1993. 2. de Groot, C. J., Luit-de Haan, G., Hulstaert, C. E., Hoomes, F. A. A patient with severe neurologic symptoms and acetoacetyl-CoA thiolase deficiency. Pediat. Res. 11: 1112-1116, 1977. 3. Laudet, V., Begue, A., Henry-Duthoit, C., Joubel, A., Martin, P., Stehelin, D., Saule, S. Genomic organization of the human thyroid hormone alpha (c-erbA-1) gene. Nucleic Acids Res. 19: 1105-1112, 1991.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ACAT2

Target: ACAT2 (Acetyl-CoA Acetyltransferase 2 (ACAT2))

Alternative Name: ACAT2

Background:

Synonyms: Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha, CAMK2A, CAMKA, KIAA0968

Tissue Specificity: High levels found in the brain, heart and lung while lower levels are seen in the liver, kidney and skeletal muscle.

Background: Acetyl-CoA acetyltransferase, cytosolic, also known as cytosolic acetoacetyl-CoA thiolase, is an enzyme that in humans is encoded by the ACAT2 (acetyl-Coenzyme A acetyltransferase 2) gene. The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 41 kDa

Gene ID: 39