RHOT2 antibody (AA 321-615)

Catalog No. ABIN7601327

This anti-RHOT2 antibody is a Rabbit Polyclonal antibody detecting RHOT2 in WB, ELISA, IHC, IP, FACS, ICC and IF. Suitable for Human, Mouse and Rat.

Quick Overview for RHOT2 antibody (AA 321-615) (ABIN7601327)

Target: RHOT2 (Ras Homolog Gene Family, Member T2 (RHOT2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RHOT2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-RHOT2 Antibody

Binding Specificity: AA 321-615

Purpose: Anti-MIRO2/RHOT2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-MIRO2/RHOT2 Antibody Picoband® (ABIN7601327). Tested in ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human;Mouse;Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MIRO2/RHOT2 recombinant protein (Position: D321-V615). Human MIRO2/RHOT2 shares 78.5% and 76.3% amino acid (aa) sequence identity with mouse and rat MIRO2/RHOT2, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry, 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunoprecipitation, 0.5-2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL
1. Fransson, A., Ruusala, A., Aspenstrom, P. Atypical Rho GTPases have roles in mitochondrial homeostasis and apoptosis. J. Biol. Chem. 278: 6495-6502, 2003. 2. Shan, Y., Hexige, S., Guo, Z., Wan, B., Chen, K., Chen, X., Ma, L., Huang, C., Zhao, S., Yu, L. Cloning and characterization of the mouse Arht2 gene which encodes a putative atypical GTPase. Cytogenet. Genome Res. 106: 91-97, 2004.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RHOT2

Target: RHOT2 (Ras Homolog Gene Family, Member T2 (RHOT2))

Alternative Name: RHOT2

Background:

Synonyms: ARHT2, C16orf39, hMiro 2, MIRO 2, miro2, Mitochondrial Rho GTPase 2, RASL, RHOT2

Background: This gene encodes a member of the Rho family of GTPases. The encoded protein is localized to the outer mitochondrial membrane and plays a role in mitochondrial trafficking and fusion-fission dynamics.

Molecular Weight: 80 kDa

Gene ID: 89941