RBP3 antibody (AA 333-1196)

Catalog No. ABIN7601363

This anti-RBP3 antibody is a Rabbit Polyclonal antibody detecting RBP3 in WB, ELISA and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for RBP3 antibody (AA 333-1196) (ABIN7601363)

Target: RBP3 (Retinol Binding Protein 3, Interstitial (RBP3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RBP3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-RBP3 Antibody

Binding Specificity: AA 333-1196

Purpose: Anti-IRBP/RBP3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-IRBP/RBP3 Antibody Picoband® (ABIN7601363). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human IRBP/RBP3 recombinant protein (Position: L333-R1196).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Carson, N. L., Simpson, N. E. A physical map of 13 markers on chromosome 10 from dosage studies on abnormal cell lines. (Abstract) Cytogenet. Cell Genet. 51: 974-975, 1989. 2. Chin, K. S., Mathew, C. G. P., Fong, S. L., Bridges, C. D. B., Ponder, B. A. J. Styl RFLP recognised by a human IRBP cDNA localised to chromosome 10. Nucleic Acids Res. 16: 1645 only, 1988. 3. Danciger, M., Kozak, C. A., Nickerson, J., Redmond, T. M., Farber, D. B. Localization of the gene for interphotoreceptor retinoid-binding protein to mouse chromosome 14 near Np-1. Genomics 8: 727-731, 1990.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RBP3

Target: RBP3 (Retinol Binding Protein 3, Interstitial (RBP3))

Alternative Name: RBP3

Background:

Synonyms: Ras-related protein Rab-6A, Rab-6, RAB6A, RAB6

Tissue Specificity: Ubiquitous.

Background: Retinol-binding protein 3, interstitial (RBP3), also known as IRBP is a protein that in humans is encoded by the RBP3 gene. Interphotoreceptor retinol-binding protein is a large glycoprotein known to bind retinoids and found primarily in the interphotoreceptor matrix of the retina between the retinal pigment epithelium and the photoreceptor cells. It is thought to transport retinoids between the retinal pigment epithelium and the photoreceptors, a critical role in the visual process.The human IRBP gene is approximately 9.5 kbp in length and consists of four exons separated by three introns. The introns are 1.6-1.9 kbp long. The gene is transcribed by photoreceptor and retinoblastoma cells into an approximately 4.3-kilobase mRNA that is translated and processed into a glycosylated protein of 135,000 Da. The amino acid sequence of human IRBP can be divided into four contiguous homology domains with 33-38 % identity, suggesting a series of gene duplication events. In the gene, the boundaries of these domains are not defined by exon-intron junctions, as might have been expected. The first three homology domains and part of the fourth are all encoded by the first large exon, which is 3,180 base pairs long. The remainder of the fourth domain is encoded in the last three exons, which are 191, 143, and approximately 740 base pairs long, respectively.

Molecular Weight: 135 kDa

Gene ID: 5949

UniProt: P10745