ATIC antibody (AA 37-592)

Catalog No. ABIN7601511

This Rabbit Polyclonal antibody specifically detects ATIC in WB, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ATIC antibody (AA 37-592) (ABIN7601511)

Target: ATIC (5-Aminoimidazole-4-Carboxamide Ribonucleotide Formyltransferase/IMP Cyclohydrolase (ATIC))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATIC antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-ATIC Antibody

Binding Specificity: AA 37-592

Purpose: Anti-ATIC Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ATIC Antibody Picoband® (ABIN7601511). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ATIC recombinant protein (Position: T37-H592).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Baresova, V., Skopova, V., Sikora, J., Patterson, D., Sovova, J., Zikanova, M., Kmoch, S. Mutations of ATIC and ADSL affect purinosome assembly in cultured skin fibroblasts from patients with AICA-ribosiduria and ADSL deficiency. Hum. Molec. Genet. 21: 1534-1543, 2012. 2. Greasley, S. E., Horton, P., Ramcharan, J., Beardsley, G. P., Benkovic, S. J., Wilson, I. A. Crystal structure of a bifunctional transformylase and cyclohydrolase enzyme in purine biosynthesis. Nature Struct. Biol. 8: 402-406, 2001. 3. Marie, S., Heron, B., Bitoun, P., Timmerman, T., Van den Berghe, G., Vincent, M.-F. AICA-ribosiduria: a novel, neurologically devastating inborn error of purine biosynthesis caused by mutation of ATIC. Am. J. Hum. Genet. 74: 1276-1281, 2004.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ATIC

Target: ATIC (5-Aminoimidazole-4-Carboxamide Ribonucleotide Formyltransferase/IMP Cyclohydrolase (ATIC))

Alternative Name: ATIC

Background:

Synonyms: POU domain, class 4, transcription factor 1

Tissue Specificity: Expressed in the brain and the retina. Present in the developing brain, spinal cord and eye.

Background: Bifunctional purine biosynthesis protein PURH is a protein that in humans is encoded by the ATIC gene. This gene encodes a bifunctional protein that catalyzes the last two steps of the de novo purine biosynthetic pathway. The N-terminal domain has phosphoribosylaminoimidazolecarboxamide formyltransferase activity, and the C-terminal domain has IMP cyclohydrolase activity. A mutation in this gene results in AICA-ribosiduria.

Molecular Weight: 65 kDa

Gene ID: 471

UniProt: P31939