KDM4A antibody (AA 379-1064)

Catalog No. ABIN7601534

This anti-KDM4A antibody is a Rabbit Polyclonal antibody detecting KDM4A in WB, IHC, ELISA and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for KDM4A antibody (AA 379-1064) (ABIN7601534)

Target: KDM4A (Lysine (K)-Specific Demethylase 4A (KDM4A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KDM4A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-KDM4A Antibody

Binding Specificity: AA 379-1064

Purpose: Anti-JHDM3A/JMJD2A/KDM4A Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-JHDM3A/JMJD2A/KDM4A Antibody Picoband® (ABIN7601534). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human JHDM3A/JMJD2A/KDM4A recombinant protein (Position: D379-E1064).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chen, Z., Zang, J., Whetstine, J., Hong, X., Davrazou, F., Kutateladze, T. G., Simpson, M., Mao, Q., Pan, C.-H., Dai, S., Hagman, J., Hansen, K., Shi, Y., Zhang, G. Structural insights into histone demethylation by JMJD2 family members. Cell 125: 691-702, 2006. 2. Ishikawa, K., Nagase, T., Suyama, M., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res. 5: 169-176, 1998. 3. Katoh, M., Katoh, M. Identification and characterization of JMJD2 family genes in silico. Int. J. Oncol. 24: 1623-1628, 2004.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for KDM4A

Target: KDM4A (Lysine (K)-Specific Demethylase 4A (KDM4A))

Alternative Name: KDM4A

Background:

Synonyms: gasdermin D, gasdermin-D

Tissue Specificity: Brain, placental, and gut tissues.

Background: Lysine-specific demethylase 4A is an enzyme that in humans is encoded by the KDM4A gene. This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor.

Molecular Weight: 150 kDa

Gene ID: 9682

UniProt: O75164

Pathways: Warburg Effect