RhoF antibody (AA 39-204)

Catalog No. ABIN7601568

This Rabbit Polyclonal antibody specifically detects RhoF in WB, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RhoF antibody (AA 39-204) (ABIN7601568)

Target: RhoF (RHOF) (Rho-related GTP-binding protein RhoF (RHOF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RhoF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-RhoF Antibody

Binding Specificity: AA 39-204

Purpose: Anti-RHOF Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RHOF Antibody Picoband® (ABIN7601568). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RHOF recombinant protein (Position: Y39-K204).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ellis, S., Mellor, H. The novel Rho-family GTPase Rif regulates coordinated actin-based membrane rearrangements. Curr. Biol. 10: 1387-1390, 2000. 2. Fan, L., Pellegrin, S., Scott, A., Mellor, H. The small GTPase Rif is an alternative trigger for the formation of actin stress fibers in epithelial cells. J. Cell Sci. 123: 1247-1252, 2010. 3. Fan, L., Yan, H., Pellegrin, S., Morigen, Mellor, H. The Rif GTPase regulates cytoskeletal signaling from plexinA4 to promote neurite retraction. Neurosci. Lett. 590: 178-183, 2015.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for RhoF

Target: RhoF (RHOF) (Rho-related GTP-binding protein RhoF (RHOF))

Alternative Name: RHOF

Background:

Synonyms: Pannexin-2, PANX2

Tissue Specificity: Expressed in fetal and adult brain. Also detected in fetal liver and skeletal muscle, but not in their adult counterparts.

Background: Rho, the Ras-related small GTPase, is responsible for the regulation of actin-based cytoskeletal structures including stress fibers, focal adhesions and the contractile ring apparatus. Rho proteins function as molecular switches that are able to turn cytokinesis on and off. Although little is known about signaling downstream of Rho, a host of putative Rho effector proteins have been described. Rho F (ras homolog gene family, member F), also known as RIF or ARHF, is a 211 amino acid membrane protein that localizes to the cytoplasmic side of the plasma membrane. Belonging to the small GTPase superfamily and the Rho family, Rho F functions cooperatively with Cdc42 and Rac to generate additional cytoskeletal structures, such as increasing variation of actin-based morphology. Rho F exists as two alternatively spliced isoforms and is encoded by a gene located on human chromosome 12q24.31.

Molecular Weight: 23 kDa

Gene ID: 54509