DIS3 antibody (AA 43-878)

Catalog No. ABIN7601729

This anti-DIS3 antibody is a Rabbit Polyclonal antibody detecting DIS3 in WB, ELISA, IHC, IF, ICC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for DIS3 antibody (AA 43-878) (ABIN7601729)

Target: DIS3 (Exosome Complex Exonuclease RRP44 (DIS3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DIS3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-DIS3 Antibody

Binding Specificity: AA 43-878

Purpose: Anti-DIS3 Antibody Picoband®

Characteristics: Anti-DIS3 Antibody Picoband® (ABIN7601729). Tested in WB, IHC, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DIS3 recombinant protein (Position: H43-K878). Human DIS3 shares 92.8% amino acid (aa) sequence identity with mouse DIS3.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry, 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gross, M. B. Personal Communication. Baltimore, Md. 6/25/2014. 2. Nagase, T., Ishikawa, K., Suyama, M., Kikuno, R., Hirosawa, M., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 6: 63-70, 1999. 3. Rozenblum, E., Vahteristo, P., Sandberg, T., Bergthorsson, J. T., Syrjakoski, K., Weaver, D., Haraldsson, K., Johannsdottir, H. K., Vehmanen, P., Nigam, S., Golberger, N., Robbins, C., and 12 others. A genomic map of a 6-Mb region at 13q21-q22 implicated in cancer development: identification and characterization of candidate genes. Hum. Genet. 110: 111-121, 2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for DIS3

Target: DIS3 (Exosome Complex Exonuclease RRP44 (DIS3))

Alternative Name: DIS3

Background: Exosome complex exonuclease RRP44 or Dis3 is an enzyme that in humans is encoded by the DIS3 gene. The DIS3 gene encodes a catalytic subunit of the exosome complex, an essential RNA degradation machinery involved in multiple aspects of RNA metabolism, including RNA processing, quality control, and turnover. DIS3 functions as an exonuclease, playing a key role in degrading various RNA species, such as messenger RNA (mRNA), ribosomal RNA (rRNA), and transfer RNA (tRNA). Its activity is crucial for maintaining cellular RNA homeostasis and gene expression regulation. Dysregulation of DIS3 expression or activity has been linked to various human diseases, including cancer and neurodegenerative disorders. Understanding the molecular mechanisms underlying DIS3 function is essential for elucidating its role in RNA metabolism and its potential implications for disease pathology and therapeutic targeting.

Molecular Weight: 109 kDa

Gene ID: 22894

UniProt: Q9Y2L1