TNNT3 antibody (AA 45-251)

Catalog No. ABIN7601762

This anti-TNNT3 antibody is a Rabbit Polyclonal antibody detecting TNNT3 in WB and ELISA. Suitable for Human, Mouse and Rat.

Quick Overview for TNNT3 antibody (AA 45-251) (ABIN7601762)

Target: TNNT3 (Fast Skeletal Troponin T (TNNT3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNNT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TNNT3 Antibody

Binding Specificity: AA 45-251

Purpose: Anti-TNNT3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TNNT3 Antibody Picoband® (ABIN7601762). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TNNT3 recombinant protein (Position: A45-K251).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Gurnett, C. A., Alaee, F., Desruisseau, D., Boehm, S., Dobbs, M. B. Skeletal muscle contractile gene (TNNT3, MYH3, TPM2): mutations not found in vertical talus or clubfoot. Clin. Orthop. Rel. Res. 467: 1195-1200, 2009. 2. Koch, A., Juan, T. S.-C., Jenkins, N. A., Gilbert, D. J., Copeland, N. G., McNiece, I. K., Fletcher, F. A. cDNA cloning and chromosomal mapping of mouse fast skeletal muscle troponin T. Mammalian Genome 8: 346-348, 1997.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for TNNT3

Target: TNNT3 (Fast Skeletal Troponin T (TNNT3))

Alternative Name: TNNT3

Background:

Synonyms: Troponin T, fast skeletal muscle, TnTf, Beta-TnTF, Fast skeletal muscle troponin T, fTnT, TNNT3

Tissue Specificity: In fetal and adult fast skeletal muscles, with a higher level expression in fetal than in adult muscle.

Background: Fast skeletal muscle troponin T (fTnT) is a protein that in humans is encoded by the TNNT3 gene. It is mapped to 11p15.5. The binding of Ca (2+) to the trimeric troponin complex initiates the process of muscle contraction. Increased Ca (2+) concentrations produce a conformational change in the troponin complex that is transmitted to tropomyosin dimers situated along actin filaments. The altered conformation permits increased interaction between a myosin head and an actin filament which, ultimately, produces a muscle contraction. The troponin complex has protein subunits C, I, and T. Subunit C binds Ca (2+) and subunit I binds to actin and inhibits actin-myosin interaction. Subunit T binds the troponin complex to the tropomyosin complex and is also required for Ca (2+)-mediated activation of actomyosin ATPase activity. There are 3 different troponin T genes that encode tissue-specific isoforms of subunit T for fast skeletal-, slow skeletal-, and cardiac-muscle. This gene encodes fast skeletal troponin T protein, also known as troponin T type 3. Alternative splicing results in multiple transcript variants encoding additional distinct troponin T type 3 isoforms. A developmentally regulated switch between fetal/neonatal and adult troponin T type 3 isoforms occurs. Additional splice variants have been described but their biological validity has not been established. Mutations in this gene may cause distal arthrogryposis multiplex congenita type 2B (DA2B).

Molecular Weight: 37 kDa

Gene ID: 7140

UniProt: P45378