ATP1A4 antibody (AA 45-680)

Catalog No. ABIN7601781

This Rabbit Polyclonal antibody specifically detects ATP1A4 in WB, FACS and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ATP1A4 antibody (AA 45-680) (ABIN7601781)

Target: ATP1A4 (ATPase, Na+/K+ Transporting, alpha 4 Polypeptide (ATP1A4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP1A4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Product Details anti-ATP1A4 Antibody

Binding Specificity: AA 45-680

Purpose: Anti-ATP1A4 Antibody Picoband®

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Anti-ATP1A4 Antibody Picoband® (ABIN7601119). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ATP1A4 recombinant protein (Position: K45-Q680). Human ATP1A4 shares 84.2% and 84.1% amino acid (aa) sequence identity with mouse and rat ATP1A4, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Buetow, K. H., Nishimura, D., Nakamura, Y., Jiang, O., Murray, J. C. A detailed multipoint gene map of chromosome 1q. Genomics 8: 13-21, 1990. Note: Erratum: Genomics 9: 564 only, 1991. 2. Jimenez, T., McDermott, J. P., Sanchez, G., Blanco, G. Na,K-ATPase alpha-4 isoform is essential for sperm fertility. Proc. Nat. Acad. Sci. 108: 644-649, 2011. 3. Keryanov, S., Gardner, K. L. Physical mapping and characterization of the human Na,K-ATPase isoform, ATP1A4. Gene 292: 151-166, 2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ATP1A4

Target: ATP1A4 (ATPase, Na+/K+ Transporting, alpha 4 Polypeptide (ATP1A4))

Alternative Name: ATP1A4

Background:

Synonyms: 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70 kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody

Tissue Specificity: Expressed in all tissues.

Background: Sodium/potassium-transporting ATPase subunit alpha-4 is an enzyme that in humans is encoded by the ATP1A4 gene. The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 4 subunit. Alternatively spliced transcript variants encoding different isoforms have been identified.

Molecular Weight: 100 kDa

Gene ID: 480

UniProt: Q13733

Pathways: Thyroid Hormone Synthesis, Proton Transport, Ribonucleoside Biosynthetic Process