Filamin A antibody (AA 451-638)

Catalog No. ABIN7601783

This anti-Filamin A antibody is a Mouse Monoclonal antibody detecting Filamin A in WB, IHC and IF. Suitable for Human.

Quick Overview for Filamin A antibody (AA 451-638) (ABIN7601783)

Target: Filamin A (FLNA) (Filamin A, alpha (FLNA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Filamin A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: 3F8

Product Details anti-Filamin A Antibody

Binding Specificity: AA 451-638

Purpose: Anti-Filamin A/FLNA Antibody Picoband® (monoclonal, 3F8)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Filamin A/FLNA Antibody Picoband® (monoclonal, 3F8) (ABIN7601783). Tested in IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Filamin A/FLNA recombinant protein (Position: E451-Q638).

Isotype: IgG1

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
1. Gorlin JB, Henske E, Warren ST, Kunst CB, D'Urso M, Palmieri G, Hartwig JH, Bruns G, Kwiatkowski DJ (October 1993). "Actin-binding protein (ABP-280) filamin gene (FLN) maps telomeric to the color vision locus (R/GCP) and centromeric to G6PD in Xq28". Genomics. 17 (2): 496-8. 2. Robertson SP, Twigg SR, Sutherland-Smith AJ, Biancalana V, Gorlin RJ, Horn D, Kenwrick SJ, Kim CA, Morava E, Newbury-Ecob R, Orstavik KH, Quarrell OW, Schwartz CE, Shears DJ, Suri M, Kendrick-Jones J, Wilkie AO (March 2003). "Localized mutations in the gene encoding the cytoskeletal protein filamin A cause diverse malformations in humans". Nat Genet. 33 (4): 487-91. 3. Gräber P, Witt HT (February 1976). "Relations between the electrical potential, pH gradient, proton flux and phosphorylation in the photosynthetic membrane". Biochimica et Biophysica Acta. 423 (2): 141-63.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Filamin A

Target: Filamin A (FLNA) (Filamin A, alpha (FLNA))

Alternative Name: FLNA

Background:

Synonyms: T-cell surface glycoprotein CD5, Lymphocyte antigen T1/Leu-1, CD5, CD5, LEU1

Tissue Specificity: Brain, liver, placenta, lymphocytes and erythrocytes.

Background: Filamin A, alpha (FLNA) is a protein that in humans is encoded by the FLNA gene. It is mapped to Xq28. The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 281 kDa

UniProt: P21333

Pathways: TCR Signaling, Maintenance of Protein Location