Pleckstrin antibody (AA 47-308)

Catalog No. ABIN7601814

This Rabbit Polyclonal antibody specifically detects Pleckstrin in WB, IHC, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for Pleckstrin antibody (AA 47-308) (ABIN7601814)

Target: Pleckstrin (PLEK)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Pleckstrin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-Pleckstrin Antibody

Binding Specificity: AA 47-308

Purpose: Anti-Pleckstrin/PLEK Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Pleckstrin/PLEK Antibody Picoband® (ABIN7601814). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Pleckstrin/PLEK recombinant protein (Position: M47-K308).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Cmarik, J. L., Hegamyer, G., Gerrard, B., Dean, M., Colburn, N. H. cDNA cloning and mapping of mouse pleckstrin (Plek), a gene upregulated in transformation-resistant cells. Genomics 66: 204-212, 2000. 2. Tyers, M., Haslam, R. J., Rachubinski, R. A., Harley, C. B. Molecular analysis of pleckstrin: the major protein kinase C substrate of platelets. J. Cell. Biochem. 40: 133-145, 1989. 3. Tyers, M., Rachubinski, R. A., Stewart, M. I., Varrichio, A. M., Shorr, R. G. L., Haslam, R. J., Harley, C. B. Molecular cloning and expression of the major protein kinase C substrate of platelets. Nature 333: 470-473, 1988.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Pleckstrin

Target: Pleckstrin (PLEK)

Alternative Name: PLEK

Background:

Synonyms: Ubiquitin carboxyl-terminal hydrolase 21, Deubiquitinating enzyme 21, Ubiquitin thioesterase 21, Ubiquitin-specific-processing protease 21, USP21, USP23, PP1490

Tissue Specificity: Highly expressed in heart, pancreas and skeletal muscle. Also expressed in brain, placenta, liver and kidney, and at very low level in lung.

Background: Enables phosphatidylinositol-3,4-bisphosphate binding activity, protein homodimerization activity, and protein kinase C binding activity. Involved in several processes, including G protein-coupled receptor signaling pathway, actin cytoskeleton organization, and positive regulation of supramolecular fiber organization. Located in cytoplasm and ruffle membrane.

Molecular Weight: 40 kDa

Gene ID: 5341

UniProt: P08567

Pathways: Inositol Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process