PTRF antibody (AA 47-390)

Catalog No. ABIN7601817

This Rabbit Polyclonal antibody specifically detects PTRF in WB, ELISA, IHC, IF, ICC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PTRF antibody (AA 47-390) (ABIN7601817)

Target: PTRF (Polymerase I and Transcript Release Factor (PTRF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTRF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-PTRF Antibody

Binding Specificity: AA 47-390

Purpose: Anti-PTRF/CAVIN1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PTRF/CAVIN1 Antibody Picoband® (ABIN7601817). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PTRF/CAVIN1 recombinant protein (Position: D47-D390).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 1-2 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hayashi, Y. K., Matsuda, C., Ogawa, M., Goto, K., Tominaga, K., Mitsuhashi, S., Park, Y.-E., Nonaka, I., Hino-Fukuyo, N., Haginoya, K., Sugano, H., Nishino, I. Human PTRF mutations cause secondary deficiency of caveolins resulting in muscular dystrophy with generalized lipodystrophy. J. Clin. Invest. 119: 2623-2633, 2009. 2. Hill, M. M., Bastiani, M., Luetterforst, R., Kirkham, M., Kirkham, A., Nixon, S. J., Walser, P., Abankawa, D., Oorschot, V. M. J., Martin, S., Hancock, J. F., Parton, R. G. PTRF-Cavin, a conserved cytoplasmic protein required for caveola formation and function. Cell 132: 113-124, 2008. 3. Jansa, P., Mason, S. W., Hoffmann-Rohrer, U., Grummt, I. Cloning and functional characterization of PTRF, a novel protein which induces dissociation of paused ternary transcription complexes. EMBO J. 17: 2855-2864, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PTRF

Target: PTRF (Polymerase I and Transcript Release Factor (PTRF))

Alternative Name: CAVIN1

Background:

Synonyms: Lymphocyte antigen 6A-2/6E-1,Ly-6A.2/Ly-6E.1,Stem cell antigen 1,SCA-1,T-cell-activating protein,TAP,Ly6a,Ly6,

Tissue Specificity: Widely expressed. .

Background: This gene encodes a protein that enables the dissociation of paused ternary polymerase I transcription complexes from the 3' end of pre-rRNA transcripts. This protein regulates rRNA transcription by promoting the dissociation of transcription complexes and the reinitiation of polymerase I on nascent rRNA transcripts. This protein also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins. This protein translocates from caveolae to the cytoplasm after insulin stimulation. Caveolae contain truncated forms of this protein and may be the site of phosphorylation-dependent proteolysis. This protein is also thought to modify lipid metabolism and insulin-regulated gene expression. Mutations in this gene result in a disorder characterized by generalized lipodystrophy and muscular dystrophy.

Molecular Weight: 50-55 kDa

Gene ID: 284119