RAPGEF3 antibody (AA 49-881)

Catalog No. ABIN7601858

This anti-RAPGEF3 antibody is a Rabbit Polyclonal antibody detecting RAPGEF3 in WB, ELISA, IF, IHC, ICC and FACS. Suitable for Human, Mouse and Monkey.

Quick Overview for RAPGEF3 antibody (AA 49-881) (ABIN7601858)

Target: RAPGEF3 (Rap Guanine Nucleotide Exchange Factor (GEF) 3 (RAPGEF3))

Reactivity: Human, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAPGEF3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-RAPGEF3 Antibody

Binding Specificity: AA 49-881

Purpose: Anti-Epac1/RAPGEF3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Epac1/RAPGEF3 Antibody Picoband® (ABIN7601858). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Epac1/RAPGEF3 recombinant protein (Position: H49-R881).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Monkey
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. de Rooij, J., Zwartkruis, F. J. T., Verheijen, M. H. G., Cool, R. H., Nijman, S. M. B., Wittinghofer, A., Bos, J. L. Epac is a Rap1 guanine-nucleotide-exchange factor ly activated by cyclic AMP. Nature 396: 474-477, 1998. 2. Dodge-Kafka, K. L., Soughayer, J., Pare, G. C., Michel, J. J. C., Langeberg, L. K., Kapiloff, M. S., Scott, J. D. The protein kinase A anchoring protein mAKAP coordinates two integrated cAMP effector pathways. Nature 437: 574-578, 2005. 3. Kawasaki, H., Springett, G. M., Mochizuki, N., Toki, S., Nakaya, M., Matsuda, M., Housman, D. E., Graybiel, A. M. A family of cAMP-binding proteins that ly activate Rap1. Science 282: 2275-2279, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RAPGEF3

Target: RAPGEF3 (Rap Guanine Nucleotide Exchange Factor (GEF) 3 (RAPGEF3))

Alternative Name: RAPGEF3

Background:

Synonyms: Fatty acid-binding protein, liver, Fatty acid-binding protein 1, Liver-type fatty acid-binding protein, L-FABP, Squalene- and sterol-carrier protein, SCP, Z-protein, p14, Fabp1

Tissue Specificity: Expressed in lung cancers, including adenocarcinoma, squamous cell carcinoma and small-cell carcinoma. Widely expressed. Expressed in fetal kidney, liver, lung and brain. In adult highest expression in heart and placenta.

Background: Rap guanine nucleotide exchange factor 3 also known as exchange factor ly activated by cAMP 1 (EPAC1) or cAMP-regulated guanine nucleotide exchange factor I (cAMP-GEFI) is a protein that in humans is encoded by the RAPGEF3 gene. As the name suggests, EPAC proteins (EPAC1 and EPAC2) are a family of intracellular sensors for cAMP, and function as nucleotide exchange factors for the Rap subfamily of RAS-like small GTPases.

Molecular Weight: 104 kDa

Gene ID: 10411

UniProt: O95398

Pathways: cAMP Metabolic Process