PRPF4 antibody (AA 5-461)
Quick Overview for PRPF4 antibody (AA 5-461) (ABIN7601886)
Target
See all PRPF4 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 5-461
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Purpose
- Anti-PRPF4 Antibody
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Cross-Reactivity (Details)
- No cross-reactivity with other proteins.
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Characteristics
- Anti-PRPF4 Antibody (ABIN7601886). Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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Purification
- Immunogen affinity purified.
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Immunogen
- E.coli-derived human PRPF4 recombinant protein (Position: R5-H461).
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Isotype
- IgG
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Application Notes
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Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry, 2-5 μg/mL,Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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Concentration
- 500 μg/mL
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Buffer
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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Storage
- 4 °C,-20 °C
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Storage Comment
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- PRPF4 (PRP4 Pre-mRNA Processing Factor 4 Homolog (PRPF4))
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Alternative Name
- PRPF4
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Background
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Synonyms: Transforming growth factor beta activator LRRC32, Garpin, Glycoprotein A repetitions predominant, GARP, Leucine-rich repeat-containing protein 32, LRRC32, D11S833E
Tissue Specificity: Heart, placenta, skeletal muscle, kidney, lung and pancreas.
Background: U4/U6 small nuclear ribonucleoprotein Prp4 is a protein that in humans is encoded by the PRPF4 gene. he protein encoded by this gene is part of a heteromeric complex that binds U4, U5, and U6 small nuclear RNAs and is involved in pre-mRNA splicing. The encoded protein also is a mitotic checkpoint protein and a regulator of chemoresistance in human ovarian cancer. Several transcript variants encoding different isoforms have been found for this gene.
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Molecular Weight
- 55 kDa
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Gene ID
- 9128
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UniProt
- O43172
Target
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