IRGM antibody (AA 50-333)

Catalog No. ABIN7601903

This anti-IRGM antibody is a Rabbit Polyclonal antibody detecting IRGM in WB and ELISA. Suitable for Mouse.

Quick Overview for IRGM antibody (AA 50-333) (ABIN7601903)

Target: IRGM (Immunity-Related GTPase Family, M (IRGM))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRGM antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-IRGM Antibody

Binding Specificity: AA 50-333

Purpose: Anti-IRGM/Irgm1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-IRGM/Irgm1 Antibody Picoband® (ABIN7601903). Tested in ELISA, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse IRGM/Irgm1 recombinant protein (Position: E50-K333).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Amre, D. K., Mack, D. R., Morgan, K., Krupoves, A., Costea, I., Lambrette, P., Grimard, G., Dong, J., Feguery, H., Bucionis, V., Deslandres, C., Levy, E., Seidman, E. G. Autophagy gene ATG16L1 but not IRGM is associated with Crohn's disease in Canadian children. Inflamm. Bowel Dis. 15: 501-507, 2009. 2. Bekpen, C., Hunn, J. P., Rohde, C., Parvanova, I., Guethlein, L., Dunn, D. M., Glowalla, E., Leptin, M., Howard, J. C. The interferon-inducible p47 (IRG) GTPases in vertebrates: Loss of the cell autonomous resistance mechanism in the human lineage. Genome Biol. 6: R92, 2005. Note: Electronic Article. 3. Brest, P., Lapaquette, P., Souidi, M., Lebrigand, K., Cesaro, A., Vouret-Craviari, V., Mari, B., Barbry, P., Mosnier, J.-F., Hebuterne, X., Harel-Bellan, A., Mograbi, B., Darfeuille-Michaud, A., Hofman, P. A synonymous variant in IRGM alters a binding site for miR-196 and causes deregulation of IRGM-dependent xenophagy in Crohn's disease. Nature Genet. 43: 242-245, 2011.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for IRGM

Target: IRGM (Immunity-Related GTPase Family, M (IRGM))

Alternative Name: Irgm1

Background:

Synonyms: NADH-ubiquinone oxidoreductase chain 2, NADH dehydrogenase subunit 2, Mtnd2, mt-Nd2, Nd2

Tissue Specificity: Ubiquitously expressed.

Background: Immunity-related GTPase family M protein (IRGM), also known as interferon-inducible protein 1 (IFI1), is an enzyme that in humans is IRGM gene. This gene encodes a member of the p47 immunity-related GTPase family. The encoded protein may play a role in the innate immune response by regulating autophagy formation in response to intracellular pathogens. Polymorphisms that affect the normal expression of this gene are associated with a susceptibility to Crohn's disease and tuberculosis. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 45 kDa

Gene ID: 15944

UniProt: Q60766