PRP19 antibody (AA 51-359)

Catalog No. ABIN7601932

The Rabbit Polyclonal anti-PRP19 antibody has been validated for WB, ELISA, FACS, IF and ICC. It is suitable to detect PRP19 in samples from Human.

Quick Overview for PRP19 antibody (AA 51-359) (ABIN7601932)

Target: PRP19 (PRPF19) (Pre-mRNA Processing Factor 19 (PRPF19))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRP19 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-PRP19 Antibody

Binding Specificity: AA 51-359

Purpose: Anti-PRPF19 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PRPF19 Antibody Picoband® (ABIN7601932). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PRPF19 recombinant protein (Position: Q51-H359).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Beck, B. D., Park, S.-J., Lee, Y.-J., Roman, Y., Hromas, R. A., Lee, S.-H. Human Pso4 is a Metnase (SETMAR)-binding partner that regulates Metnase function in DNA repair. J. Biol. Chem. 283: 9023-9030, 2008. 2. Gross, M. B. Personal Communication. Baltimore, Md. 9/6/2016. 3. Mahajan, K. N., Mitchell, B. S. Role of human Pso4 in mammalian DNA repair and association with terminal deoxynucleotidyl transferase. Proc. Nat. Acad. Sci. 100: 10746-10751, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PRP19

Target: PRP19 (PRPF19) (Pre-mRNA Processing Factor 19 (PRPF19))

Alternative Name: PRPF19

Background:

Synonyms: PH domain leucine-rich repeat-containing protein phosphatase 1, Pleckstrin homology domain-containing family E member 1, PH domain-containing family E member 1, Suprachiasmatic nucleus circadian oscillatory protein, hSCOP, PHLPP1, KIAA0606, PHLPP, PLEKHE1, SCOP

Tissue Specificity: Monocyte/macrophage specific.

Background: Pre-mRNA-processing factor 19 is a protein that in humans is encoded by the PRPF19 gene. Enables identical protein binding activity and ubiquitin-ubiquitin ligase activity. Involved in several processes, including DNA damage checkpoint signaling, cellular protein metabolic process, and mRNA splicing, via spliceosome. Acts upstream of or within protein polyubiquitination. Located in cytoplasm, nuclear speck, and site of double-strand break. Part of Prp19 complex and U2-type catalytic step 2 spliceosome. Colocalizes with DNA replication factor A complex.

Molecular Weight: 54 kDa

Gene ID: 27339

Pathways: Ribonucleoprotein Complex Subunit Organization